Thermo Scientific - Pierce Protein Research Products



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Application Notes
Affinity Purification
Sample Preparation
Protein Assays
Western Blotting/ELISA/Immunohistochemistry
Protein Labeling
Crosslinking
Nucleic Acid Labeling and Detection
GC/HPLC Derivatization

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YOU ARE HERE:  Tech Tips  


Tech Tips

Application Notes 
Acquiring chemiluminescent and fluorescent images using the Kodak Image Station 2000MM (Application Note #13)


Albumin removal and analysis with Pierce Blue Albumin Removal Kit and Agilent 2100 Bioanalyzer (Application Note #1)


Biotinylated DNA binding capacities of Pierce Avidin Supports (Application Note #14)


Immunoblot imaging with the ChemiImager 4000 Cooled CCD Camera and SuperSignal West Chemiluminescent Substrates (Application Note #5)


Experiments using the North2South Chemiluminescent Detection System for nucleic acid blotting (Application Note #6)


Inhibitory effects of C3 exoenzyme on Rho activation using Active GTPase Pull-Down Kits (Application Note #8)


Comparison of filter-based with laser-based fluorescence imaging of Western blots on PVDF membrane (Application Note #10)


Factors that cause the appearance of ghost bands when using chemiluminescent detection systems in a Western blot (Application Note #11)


Acquiring chemiluminescent blot images with the Eagle Eye II still video system (Application Note #4)


Methods and considerations for quantitative Western blotting using SuperSignal Chemiluminescent Substrates (Application Note #12)


Affinity Purification 
Batch and spin cup methods for affinity purification of proteins (Tech Tip #4)


Remove air bubbles from columns to restore flow rate (Tech Tip #7)


Prepare molecules with poor solubility for immobilization on affinity supports (Tech Tip #12)


Pack beaded affinity resin into columns (Tech Tip #13)


Prepare a reversible and measurable sulfhydryl-reactive affinity column (Tech Tip #26)


Optimize elution conditions for immunoaffinity purification (Tech Tip #27)


Degas buffers for use in affinity and gel filtration columns (Tech Tip #29)


Binding characteristics of Protein A, Protein G, Protein A/G and Protein L (Tech Tip #34)


Protein stability and storage (Tech Tip #43)


Remove BSA and gelatin from antibody solutions using Melon Gel (Tech Tip #55)


Ion exchange chromatography (Tech Tip #62)


Immunoprecipitation (IP) technical guide and protocols (Tech Tip #64)


Sample Preparation 
Remove detergent from protein samples (Tech Tip #19)


Dialysis: an overview (Tech Tip #20)


Convert between times gravity (x g) and centrifuge rotor speed (RPM)(Tech Tip #40)


Protein stability and storage (Tech Tip #43)


Compare Pierce molecular weight markers for relative migration in Precise Protein Gels (Tech Tip #47)


Compare Precise Protein Gels for electrophoretic resolution of Pierce molecular weight markers (Tech Tip #48)


Acetone precipitation of proteins (Tech Tip #49)


Process stained polyacrylamide gel pieces for mass spectrometry (Tech Tip #50)


Purify proteins from polyacrylamide gels (Tech Tip #51)


Pierce Spin Cup and Column dimensions and volumes (Tech Tip #52)


Pierce Concentrator dimensions (Tech Tip #53)


Slide-A-Lyzer Dialysis Cassette and MINI Unit dimensions (Tech Tip #54)


Calculate reagent log P values to determine solubility characteristics (Tech Tip #56)


Prepare SILAC peptides using the In-Gel Tryptic Digestion Kit (Tech Tip #60)


Ion exchange chromatography (Tech Tip #62)


Slide-A-Lyzer MINI Dialysis Unit applications guide.


Protein Assays 
Extinction coefficients guide (Tech Tip #6)


Eliminate interfering substances from samples for BCA Protein Assay (Tech Tip #8)


Quantitate immobilized protein (Tech Tip #9)


Determine acceptable wavelengths for measuring protein assays (Tech Tip #25)


How to use a protein assay standard curve (Tech Tip #57)


Western Blotting/ELISA/Immunohistochemistry 
Guide to enzyme substrates for Western blotting (Tech Tip #32)


Guide to enzyme substrates for ELISA (Tech Tip #33)


Spike-and-recovery and linearity-of-dilution assessment (Tech Tip #58)


Choosing a secondary antibody: A guide to fragment specificity (Tech Tip #59)


De-paraffinize tissue samples for staining or immunohistochemical procedures (Tech Tip #61)


Chemiluminescent Western blotting technical guide and protocols (Tech Tip #67)


Block endogenous biotin (Tech Tip #16)


Convert to SuperSignal West Pico Substrate from ECL Substrate (Tech Tip #21)


Determine source of nonspecific background signal in Western blots detected using SuperSignal Chemiluminescent Substrates (Tech Tip #22)


Strip and reprobe Western blots (Tech Tip #23)


Optimize antigen and antibody concentrations for Western blots (Tech Tip #24)


Protein stability and storage (Tech Tip #43)


Immunoprecipitation on Protein A- or Protein G-Coated Plates for Western Blot Analysis


Protein Labeling 
Perform labeling and other reactions in Slide-A-Lyzer Dialysis Cassettes (Tech Tip #14)


Biotinylate carboxyl groups with EDC and Biotin Hydrazide (Tech Tip #15)


Block endogenous biotin (Tech Tip #16)


Calculate dye:protein (F/P) molar ratios (Tech Tip #31)


Preferentially biotinylate N-terminal alpha-amino groups in peptides (Tech Tip #46)


Crosslinking 
Attach a protein onto glass, silica or quartz surface using a cleavable crosslinker (Tech Tip #1)


Attach a protein onto a gold surface (Tech Tip #2)


Determine reactivity of NHS ester biotinylation and crosslinking reagents (Tech Tip #3)


Attach an antibody onto glass, silica or quartz surface (Tech Tip #5)


Light sources and conditions for photoactivation of aryl azide crosslinking reagents (Tech Tip #11)


Block amino groups to prevent polymer formation in peptide-carrier protein conjugations (Tech Tip #18)


Nucleic Acid Labeling and Detection 
Strip Northern and Southern blots (Tech Tip #28)


Modify and label oligonucleotide 5´ phosphate groups (Tech Tip #30)


Electrophorese DNA in Precise Protein Gels (Tech Tip #44)


Anneal complementary pairs of oligonucleotides (Tech Tip #45)


GC/HPLC Derivatization 
Surface treatment reagents (Tech Tip #36)


Silylation guide for derivatization of functional groups (Tech Tip #37)


Drugs-of-abuse derivatization reagent guide and references (Tech Tip #38)


Learn more about other Thermo Scientific Life Science Research Products
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