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Thermo Scientific - Pierce Protein Research Products


	Application Notes
	Affinity Purification
	Sample Preparation
	Protein Assays
	Western Blotting/ELISA/Immunohistochemistry
	Protein Labeling
	Crosslinking
	Nucleic Acid Labeling and Detection
	GC/HPLC Derivatization

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Home > Tech Tips

Tech Tips

Application Notes

Acquiring chemiluminescent and fluorescent images using the Kodak Image Station 2000MM (Application Note #13)

Albumin removal and analysis with Pierce Blue Albumin Removal Kit and Agilent 2100 Bioanalyzer (Application Note #1)

Biotinylated DNA binding capacities of Pierce Avidin Supports (Application Note #14)

Immunoblot imaging with the ChemiImager 4000 Cooled CCD Camera and SuperSignal West Chemiluminescent Substrates (Application Note #5)

Experiments using the North2South Chemiluminescent Detection System for nucleic acid blotting (Application Note #6)

Inhibitory effects of C3 exoenzyme on Rho activation using Active GTPase Pull-Down Kits (Application Note #8)

Comparison of filter-based with laser-based fluorescence imaging of Western blots on PVDF membrane (Application Note #10)

Factors that cause the appearance of ghost bands when using chemiluminescent detection systems in a Western blot (Application Note #11)

Acquiring chemiluminescent blot images with the Eagle Eye II still video system (Application Note #4)

Methods and considerations for quantitative Western blotting using SuperSignal Chemiluminescent Substrates (Application Note #12)

Affinity Purification

Batch and spin cup methods for affinity purification of proteins (Tech Tip #4)

Remove air bubbles from columns to restore flow rate (Tech Tip #7)

Prepare molecules with poor solubility for immobilization on affinity supports (Tech Tip #12)

Pack beaded affinity resin into columns (Tech Tip #13)

Prepare a reversible and measurable sulfhydryl-reactive affinity column (Tech Tip #26)

Optimize elution conditions for immunoaffinity purification (Tech Tip #27)

Degas buffers for use in affinity and gel filtration columns (Tech Tip #29)

Binding characteristics of Protein A, Protein G, Protein A/G and Protein L (Tech Tip #34)

Protein stability and storage (Tech Tip #43)

Remove BSA and gelatin from antibody solutions using Melon Gel (Tech Tip #55)

Ion exchange chromatography (Tech Tip #62)

Immunoprecipitation (IP) technical guide and protocols (Tech Tip #64)

Sample Preparation

Remove detergent from protein samples (Tech Tip #19)

Dialysis: an overview (Tech Tip #20)

Convert between times gravity (x g) and centrifuge rotor speed (RPM)(Tech Tip #40)

Protein stability and storage (Tech Tip #43)

Compare Pierce molecular weight markers for relative migration in Precise Protein Gels (Tech Tip #47)

Compare Precise Protein Gels for electrophoretic resolution of Pierce molecular weight markers (Tech Tip #48)

Acetone precipitation of proteins (Tech Tip #49)

Process stained polyacrylamide gel pieces for mass spectrometry (Tech Tip #50)

Purify proteins from polyacrylamide gels (Tech Tip #51)

Pierce Spin Cup and Column dimensions and volumes (Tech Tip #52)

Pierce Concentrator dimensions (Tech Tip #53)

Slide-A-Lyzer Dialysis Cassette and MINI Unit dimensions (Tech Tip #54)

Calculate reagent log P values to determine solubility characteristics (Tech Tip #56)

Prepare SILAC peptides using the In-Gel Tryptic Digestion Kit (Tech Tip #60)

Ion exchange chromatography (Tech Tip #62)

Slide-A-Lyzer MINI Dialysis Unit applications guide.

Protein Assays

Extinction coefficients guide (Tech Tip #6)

Eliminate interfering substances from samples for BCA Protein Assay (Tech Tip #8)

Quantitate immobilized protein (Tech Tip #9)

Determine acceptable wavelengths for measuring protein assays (Tech Tip #25)

How to use a protein assay standard curve (Tech Tip #57)

Western Blotting/ELISA/Immunohistochemistry

Guide to enzyme substrates for Western blotting (Tech Tip #32)

Guide to enzyme substrates for ELISA (Tech Tip #33)

Spike-and-recovery and linearity-of-dilution assessment (Tech Tip #58)

Choosing a secondary antibody: A guide to fragment specificity (Tech Tip #59)

De-paraffinize tissue samples for staining or immunohistochemical procedures (Tech Tip #61)

ELISA technical guide and protocols (Tech Tip #65)

Chemiluminescent Western blotting technical guide and protocols (Tech Tip #67)

Block endogenous biotin (Tech Tip #16)

Convert to SuperSignal West Pico Substrate from ECL Substrate (Tech Tip #21)

Determine source of nonspecific background signal in Western blots detected using SuperSignal Chemiluminescent Substrates (Tech Tip #22)

Strip and reprobe Western blots (Tech Tip #23)

Optimize antigen and antibody concentrations for Western blots (Tech Tip #24)

Protein stability and storage (Tech Tip #43)

Immunoprecipitation on Protein A- or Protein G-Coated Plates for Western Blot Analysis

Protein Labeling

Perform labeling and other reactions in Slide-A-Lyzer Dialysis Cassettes (Tech Tip #14)

Biotinylate carboxyl groups with EDC and Biotin Hydrazide (Tech Tip #15)

Block endogenous biotin (Tech Tip #16)

Calculate dye:protein (F/P) molar ratios (Tech Tip #31)

Preferentially biotinylate N-terminal alpha-amino groups in peptides (Tech Tip #46)

Crosslinking

Attach a protein onto glass, silica or quartz surface using a cleavable crosslinker (Tech Tip #1)

Attach a protein onto a gold surface (Tech Tip #2)

Determine reactivity of NHS ester biotinylation and crosslinking reagents (Tech Tip #3)

Attach an antibody onto glass, silica or quartz surface (Tech Tip #5)

Light sources and conditions for photoactivation of aryl azide crosslinking reagents (Tech Tip #11)

Block amino groups to prevent polymer formation in peptide-carrier protein conjugations (Tech Tip #18)

Nucleic Acid Labeling and Detection

Strip Northern and Southern blots (Tech Tip #28)

Modify and label oligonucleotide 5´ phosphate groups (Tech Tip #30)

Electrophorese DNA in Precise Protein Gels (Tech Tip #44)

Anneal complementary pairs of oligonucleotides (Tech Tip #45)

GC/HPLC Derivatization

Surface treatment reagents (Tech Tip #36)

Silylation guide for derivatization of functional groups (Tech Tip #37)

Drugs-of-abuse derivatization reagent guide and references (Tech Tip #38)

Learn more about other Thermo Scientific Life Science Research Products

Biopolymers	Cell Culture	High Content Screening
Nucleic Acid Technologies	PCR Reagents & Plastics	RNAi Technologies	European Sales

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