Introducing a fast, sensitive and consistent coomassie protein stain
Thermo Scientific Imperial Protein Stain is a ready-to-use coomassie stain for detecting protein bands in SDS-PAGE and 2-D gels, detecting as little as 6ng of protein in just 20 minutes. Even greater sensitivity (i.e., 3ng) can be achieved with longer staining and water wash times.
Highlights of Imperial Protein Stain:
Outstanding Performance
- Sensitive: > 3ng protein/band
- Fast – detect as little as 6ng protein/band in just 20 minutes
- Robust – highly consistent, reproducible protein staining
- Excellent photo-documentation – photographs/scans better than other coomassie stains
- Versatile – Compatible with mass spec analysis and protein sequencing
Convenience
- Destain background with water
- No fixation step required
- Ready-to-use reagent
- Stable – store on your bench top for up to one year
- Flexible – adjust staining and washing times to meet demanding time/sensitivity requirements
About Imperial Protein Stain:
Imperial Protein stain is a unique formulation of Coomassie Brilliant Blue R-250 that delivers substantial improvements in protein-staining performance compared to homemade or other commercially available coomassie-based stains. The easy-to-follow protocol is flexible to meet demanding time and sensitivity requirements.
The use of Imperial Protein Stain does not result in the problems (e.g., staining consistency) that can be associated with other coomassie G-250 stain preparations. In addition to faster protein band development and improved sensitivity over standard coomassie G-250 stains, Imperial Protein Stain does not require methanol/acetic acid fixation and destaining, saving valuable preparation time and minimizing reagent cost.
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| Thermo Scientific Imperial Protein Stain Protocol |
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| Thermo Scientific Imperial Protein Stain is fast and sensitive. Proteins were separated on Novex 4-20% Tris-glycine gels, stained for 5 minutes and destained 3 x 5 minutes in water. Lane 1. BSA only (6µg), Lanes 2-9. contained the indicated proteins at the following concentrations: Lane 2: 1,000ng, Lane 3. 200ng, Lane 4. 100ng, Lane 5. 50ng, Lane 6. 25ng, Lane 7. 12ng, Lane 8. 6ng and Lane 9. 3ng. |
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| Enhanced sensitivity and crystal-clear background using Imperial Protein Stain. For even greater sensitivity and reduced background, gels can be stained with Imperial Protein Stain for 1 hour and washed with water from 1 hour to overnight. Lane 1: BSA only (6µg), Lane 2: 1000ng, Lane 3: 200ng, Lane 4: 100ng, Lane 5: 50ng, Lane 6: 25ng, Lane 7: 12ng, Lane 8: 6ng, Lane 9: 3ng |
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