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Protein Interactions  |
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Information about chromatin IP (chromatin immunoprecipitation), including the assay steps and recommended reagents for the procedure.
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Convenient kits to affinity purify protein interaction binding complexes via co-immunoprecipitation (co-IP) or non-antibody affinity system (Pull-Down).
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Evaluate protein interactions by probing in-gel or on-membrane with biotin-labeled or tagged binding proteins which can be detected secondarily with an HRP-conjugate and sensitive substrate system.
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The Protein Interaction Mapping Kit contains the FeBABE protein cutting reagent, very useful in mapping interaction sites of known proteins.
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Reference guide to in vivo crosslinking and compatible in vivo crosslinkers for investigating cell surface or intracellular protein interactions.
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Biotin-labeled or iodinatable heterobifunctional, cleavable crosslinkers that can be used to tag a bait protein and then transfer the tag onto an interacting protein following a binding interaction.
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Photo-Leucine and Photo-Methionine are amino acid analogs that can be incorporated into proteins in actively growing cells. In vivo protein interactions are covalently crosslinked upon UV-light activation of the diazirine moiety.
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Compact, 8-Watt ultraviolet lamp for activating aryl azide and diazirine groups in certain photo-reactive reagents to initiate crosslinking between interacting proteins. Long, midrange, and short wavelength settings allow use with a many techniques.
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Protein transfection reagent, Small GTPase pull-down kits (Rho, Ras, Rac1, Cdc42, Rap1) and plate-based transcription factor assay kits (NFkB and c-Fos).
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