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Columns to handle a wide variety of resin volumes for affinity purification!
Thermo Scientific Pierce Centrifuge Columns are convenient tools for handling 40 µl - 10 ml of an affinity support for purification. Simply add the affinity resin to one of the polypropylene columns, remove the twist-off bottom and allow the resin to pack itself. Then add your sample and allow it to bind to the support and use a centrifuge to efficiently wash away any contaminants and elute your purified protein.
Pierce Centrifuge Columns can be used in either spin or gravity-flow format. The spin format reduces sample processing time and makes column washing and elution more efficient. For gravity-flow procedures and when slow processing time is desired for binding steps with dilute samples, Column Extenders can be used to increase the column reservoir capacity. Use the any combination of processing techniques to get the best possible chromatography results!
Centrifuge columns are made from low protein binding polypropylene for compatibility with protein purification, and fit into standard centrifuge tubes for use in any centrifuge.
Applications for Centrifuge Columns
Affinity purification / Affinity chromatography
Immunodepletion
Spin desalting
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0.8 ml Pierce Centrifuge Columns
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0.8 ml Centrifuge Columns
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Total volume 800 µl
Resin volume 40-400 µl
Filter type, pore size: Polyethylene filter, ~30 µm pore size
Receiver tube: Fits standard microcentrifuge tubes
(e.g., #69720)
Caps: O-ring screw-top cap
Twist-off bottom
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2 ml Pierce Centrifuge Columns
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2 ml Centrifuge Columns
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Total volume: 5 ml (2 ml resin bed, 3 ml reservoir)
Resin volume: 2 ml
Filter type, pore size: Polyethylene filter, ~30 µm pore size
Receiver tube: Fits standard 15 ml conical centrifuge tubes
Caps: Screw-top cap
Twist-off bottom and press-on cap to reseal
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5 ml Pierce Centrifuge Columns
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5 ml Centrifuge Columns
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Total volume: 8 ml (5 ml resin bed, 3 ml reservoir)
Resin volume: 5 ml
Filter type, pore size: Polyethylene filter, ~30 µm pore size
Receiver tube: Fits standard 15 ml conical centrifuge tubes
Caps: Screw-top cap
Twist-off bottom and press-on cap to reseal
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10 ml Pierce Centrifuge Columns
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10 ml Centrifuge Columns
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Total volume: 22 ml (10 ml resin bed, 12 ml reservoir)
Resin volume: 10 ml
Filter type, pore size: Polyethylene filter, ~30 µm pore size
Receiver tube: Fits standard 50 ml conical centrifuge tubes
Caps: Screw-top cap
Twist-off bottom and press-on cap to reseal
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Column Extenders for Pierce Centrifuge Columns
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Column Extenders
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Increases column reservoir volume by 35-40 ml
Designed especially for use in gravity-flow procedures
Fits 2 ml, 5 ml and 10 ml Pierce Centrifuge Columns
Made of polypropylene
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Related Products
Additional microcentrifuge columns for affinity chromatography
Gravity-flow columns for affinity chromatography
Affinity chromatography supports
Immunoprecipitation kits
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Bulk quantities: Inquire
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