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Thermo Scientific - Pierce Protein Research Products  
Coupling through Amines
Coupling through Carbohydrates
Coupling through Carboxyl Groups
Coupling through Sulfhydryls
Coupling through Active Hydrogens
Polystyrene Beads

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YOU ARE HERE:  Browse Product Catalog > Affinity Purification > Supports to Immobilize Proteins  



Supports to Immobilize Proteins 
Activated supports to immobilize any ligand (antibodies, glycoproteins, other proteins, peptides, etc.) to create an affinity resin capable of purifying anything to which the immobilized ligand has an affinity.

Coupling through Amines 
Activated agarose beads and other resins for covalent immobilization through primary amines , which are abundant on proteins (N-terminus and side chain of lysine residues). Convenient kits use chemistries superior to cyanogen bromide (CNBr) activation.  


Coupling through Carbohydrates 
Hydrazide-activated agarose beads and UltraLink Resin for covalent immobilization of glycoproteins. Mild oxidation of carbohydrates creates aldehydes that react spontaneously with the activated resin. Excellent for polyclonal antibody immobilization. 


Coupling through Carboxyl Groups 
Long-arm, amine-activated agarose beads and UltraLink Resin for covalent immobilization of carboxyl-containing molecules (e.g., C-terminus of peptides) using the carbodiimide crosslinker EDC. Complete Kits include resin, columns and crosslinker. 


Coupling through Sulfhydryls 
Long-arm, iodoacetyl-activated agarose beads and UltraLink Resin for covalent attachment of sulfhydryl-molecules. Immobilize cysteine-terminated peptide antigens for use in antibody purification. Large- and small-scale protein and peptide kits offered. 


Coupling through Active Hydrogens 
PharmaLink Immobilization Kit containing DADPA(amine)-activated agarose beads and formaldehyde coupling agent to covalently immobilize steroid, drug, and other small organic compounds that have active hydrogen atoms as their only available reactive group. 


Polystyrene Beads 
Pellet-sized (0.25 inch diameter) beads. Underivatized beads for direct protein adsorption. Hydrazide-activated beads for coupling through aldehydes or oxidized carbohydrate groups. Special finish provides large bead surface area. 

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