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Thermo Scientific DyLight 680B (B=brighter) – Thermo Scientific DyLight 680B Dye is a derivative of the original DyLight 680 Dye, which is a near-infrared (NIR) dye commonly used with the *LI-COR Odyssey Imaging System and for small animal in vivo imaging. The excitation and emission wavelengths displayed by DyLight 680B Dye are similar to DyLight 680 Dye and *Alexa Fluor 680 Dye; however DyLight 680B Dye exhibits fluorescence intensity up to 10 times greater than Alexa Fluor 680 Dye.
• DyLight 680B Amine Reactive Dye
• DyLight 680B Sulfhydryl Reactive Dye
• DyLight 680B Antibody Labeling Kits
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Feb 2010 |
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Pierce Fast Western Blot Kit, SuperSignal West Femto Substrate – The Thermo Scientific Pierce Fast Western Blot Kits are now available with SuperSignal West Femto, the most sensitive Pierce Fast Western Blotting kit and chemiluminescent substrate system available.. These kits are optimized with SuperSignal West Femto Substrate to probe and develop Western blots in about 1 hour with mid to low femtogram levels of detection.
• All optimized reagents included
• Kits for 2 or 10 blots (8 x 10 cm) available
• Requires only mouse or rabbit primary antibodies for target protein
When used with the Pierce Fast Semi-Dry Blotter, mini protein gels can be transferred and developed in under 3 hours!
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Jan 2010 |
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Pierce Fast Western Blot Kit, SuperSignal West Dura Substrate – The Thermo Scientific Pierce Fast Western Blot Kits are now available with SuperSignal West Dura Extended Duration Chemiluminescent Western Blot Substrate. This kit is optimized to develop a Western blot in about 1 hour with femtogram levels of detection. No vacuum or special equipment is required and ideal for use with CCD imaging systems.
• All optimized reagents included
• Kits for 2 or 10 blots (8 x 10 cm) available
• Requires only mouse or rabbit primary antibodies for target protein
When used with the Pierce Fast Semi-Dry Blotter, mini protein gels can be transferred and developed in under 3 hours!
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Jan 2010 |
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Pierce IP Lysis Buffer – The Thermo Scientific Pierce IP Lysis Buffer is a mammalian whole cell lysis buffer based on a modified RIPA buffer formulation without SDS. This moderate strength lysis buffer is able to effectively solubilize cellular proteins but does not liberate genomic DNA or disrupt protein complexes like RIPA buffer. The Pierce IP Lysis Buffer is especially formulated for pulldown and immunoprecipitation assays and as a wash buffer for affinity resins.
• Optimized for immunoprecipitation and pull-down assays
• Compatible with protein, reporter and immuno assay procedures
• Gentle formulation helps maintain protein complexes
Browse all Thermo Scientific Pierce Cell Lysis Reagents
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Dec 2009 |
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NHS-Activated Agarose – Thermo Scientific Pierce NHS-Activated Agarose Resin allows simple and efficient covalent immobilization of proteins to a beaded-agarose support, providing a valuable tool for affinity purification of antibodies, antigens or other biomolecules. The coupling reaction is performed in an amine-free buffer at pH 7-9. Coupling efficiency is typically greater than 80%, regardless of the ligands molecular weight or pI.
• Rapid Immobilization
• Bulk immobilization
• Immobilization of ligands for purification of recombinant proteins
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Dec 2009 |
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Pierce C18 Tips – Thermo Scientific Pierce C18 Tips enable fast and efficient capture, concentration, desalting and elution of peptides and proteins. The five-step procedure is simple and requires less than 5 minutes to process protein digests, strong cation exchange fractions and other protein and peptide samples for mass spectrometric (MS) analyses.
• Fast-flow tips have improved flow over other commercially available tips
• High-binding capacity in a convenient tip format
• Two pipette tip formats for processing up to 8µg or 80µg of sample
• Eight packs and 96-tip trays are automation compatible
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Nov 2009 |
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Target-Specific In-Cell ELISA Kits – The Thermo Scientific Pierce In-Cell ELISA Kits are now available as complete kits for the detection of specific intracellular targets. Each Pierce In-Cell ELISA comes with specific antibodies and other required reagents to detect two targets and normalize relative protein levels. Colorimetric and Near IR detection kits are available for the following proteins:
• AKT, Phospho AKT
• EGFR, Phospho EGFR
• ERK1/2, Phospho ERK1/2
• GSK3α/β, Phospho GSK3α/β
• S6, Phospho S6
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• STAT3, Phospho STAT3
• STAT6, Phospho STAT6
• Cleaved Caspase 3, Tubulin
• p53, Tubulin
• Cleaved PARP, Tubulin
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Nov 2009 |
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Human In Vitro Protein Expression Kit – Thermo Scientific Pierce Human In Vitro Protein Expression Kits are complete systems for in vitro transcription and translation of proteins from DNA or mRNA templates from a mixture of a specialized human cell lysate and optimized supplements.
• Accurate – generate full-length, functional recombinant protein
• High yield – biosynthesize proteins up to 40 µg/ml concentration
• Versatile – express proteins from any organism
The optimized cloning and in vitro transcription vector (T7 Cell-Free Expression Vector (pT7CFE1-CHis)) recommended for use with this system is also available.
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Nov 2009 |
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Restore Fluorescent Western Blot Stripping Buffer – The Thermo Scientific Restore Fluorescent Western Blot Stripping Buffer is a gentle and highly effective for quickly removing primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots from PVDF membranes.
• Fast – strip blots in only 15 minutes at room temperature
• Efficient – effectively strips blots the first time
• Economical – less expensive than other commercially available products
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Nov 2009 |
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Pierce Detergent Removal Spin Columns – Thermo Scientific Pierce Detergent Removal Spin Columns efficiently remove high concentrations of detergents from 10µl to 1ml of sample with minimal sample loss. The detergent affinity resin specifically binds a wide variety of detergents and surfactants that are commonly used in protein extraction and biological sample preparation.
• High protein recovery
• Four sizes of pre-packed spin columns available
• Treats samples from 10µl to 1ml
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Nov 2009 |
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Zeba Spin Desalting Columns, 40K MWCO – Thermo Scientific Zeba Desalting Columns are now available with a 40K MWCO gel filtration resin. These desalting columns have excellent desalting properties and high protein recovery like of 7K MWCO Zeba Products, but also allow removal of larger contaminants.
• Currently available in 4 sizes for sample volumes 0.2ml to 4ml
• Zeba micro format (samples 7µl to 200µl) coming soon!
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Oct 2009 |
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Pierce Fast Western Blot Kit, SuperSignal West Pico Substrate – The Thermo Scientific Pierce Fast Western Blot Kits are now available with SuperSignal West Pico Chemiluminescent Western Blot Substrate. This kit is optimized to develop a Western blot in about 1 hour with picogram levels of detection. No vacuum or special equipment is required.
• All optimized reagents included
• Kits for 5 or 25 blots (8 x 10 cm) available
• Requires only mouse or rabbit primary antibodies for target protein
When used with the Pierce Fast Semi-Dry Blotter, mini protein gels can be transferred and developed in under 3 hours!
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Oct 2009 |
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New 150K MWCO Pierce Protein Concentrators – The newest member of the Thermo Scientific Pierce Concentrators. Available in packs of 10 and 25 concentrators. These .
• 9K MWCO, 7ml and 20 ml sizes
• 20K MWCO, 7ml and 20 ml sizes
• 150K MWCO, 7ml and 20 ml sizes
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Oct 2009 |
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DyLight 350 Conjugated Antibodies – The newest additions to the high-intensity, photostable DyLight conjugate family, the DyLight 350 conjugated polyclonal antibodies are excellent alternatives to Alexa* Fluor 350 and AMCA as molecular probes for use in fluorescence microscopy, flow cytometry, Western blotting, ELISA, high-content screening and other array platforms.
• Goat Anti-Mouse (H&L), DyLight 350 Conjugated
• Goat Anti-Rabbit (H&L), DyLight 350 Conjugated
• Goat Anti-Mouse (H&L), Highly Cross-Adsorbed, DyLight 350 Conjugated
• Goat Anti-Rabbit (H&L), Highly Cross-Adsorbed, DyLight 350 Conjugated
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Sept 2009 |
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Pierce Protein Concentrators – The popular iCON Concentrators are now the Thermo Scientific Pierce Concentrators. Available in a 25 pack and new 10 pack size.
• 9K MWCO, 7ml and 20 ml sizes
• 20K MWCO, 7ml and 20 ml sizes
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Aug 2009 |
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Pierce Western Blotting Buffers in 5L economy size – Popular Thermo Scientific Pierce Western Blotting buffers are now available in large, economical 5L buffer boxes.
• Restore Western Blot Stripping Buffer, 5L
• Restore PLUS Western Blot Stripping Buffer, 5L
• 10X Pierce Western Blot Transfer Buffer, Methanol-free, 5L
• SuperBlock Blocking Buffer in PBS, 5L
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Aug 2009 |
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Slide-A-Lyzer G2 Dialysis Cassettes – Thermo Scientific Slide-A-Lyzer G2 Dialysis Cassette offers maximum efficiency, convenience and sample protection in one package. Sample loading and removal are easily accomplished by using a serological pipette or hypodermic needle (optional) attached to a syringe. The built-in air chamber provides sample buoyancy and vertical orientation of the cassette during dialysis.
• Pipette-accessible for easy sample loading and retrieval
• Self-floating chambers for buoyancy and vertical orientation during dialysis
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Aug 2009 |
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DyLight 350 NHS Ester – Thermo Scientific DyLight grows larger with the addition of the DyLight 350. DyLight 350 is the 10th member in this family of high-intensity, photostable fluorescent dyes for labeling antibodies and other molecular probes for use in fluorescence microscopy, flow cytometry, Western blotting, ELISA, high-content screening and other array platforms.
• DyLight 350 replace Alexa* Flour 350 Dye and AMCA
• DyLight Fluors have improved photostability and water solubility
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July 2009 |
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Pierce Fast Western System – Thermo Scientific Pierce Fast Transfer System reduces protein transfer time from SDS-PAGE to a membrane from 45 minutes to only 10 minutes. The system consists of two parts, the Pierce Fast Semi-Dry Transfer Buffer and the Pierce Fast Semi-Dry Blotter.
• Cost effective using less consumables than competition fast system
• Environmentally friendly formulation required no methanol
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July 2009 |
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High Sensitivity Biotin-Binding Microplates – Thermo Scientific Pierce High Sensitivity Streptavidin and NeutrAvidin Coated Plates enable detection of low concentrations of biotinylated antibodies and other proteins (i.e., 5 ng/ml). All plates are pre-blocked with Blocker BSA.
• Streptavidin Coated 8-Well strip plates, clear and black
• NeutrAvidin Coated 8-Well strip plates, clear and black
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July 2009 |
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Fluorescence Biotin Quantitation Kit – The Thermo Scientific Fluorescence Biotin Quantitation Kit requires only 10 µl of sample to accurately measure the biotinylation level of biomolecules. This product is more accurate than the leading competitor's.
• Economical
• Fast
• Sensitive
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June 2009 |
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DyLight Labeled Highly Cross-Adsorbed Secondary Antibodies – DyLight Labeled Highly Cross-Adsorbed Secondary Antibodies show minimum cross-reactivity and are suitable for multiplex experiments. They are designed to reduce nonspecific background staining and offer high specificity, low background and increased assay sensitivity.
• ELISA
• Flow cytometry
• Protein Arrays
• Fluorescence microscopy
• High-content analysis
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Apr 2009 |
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SH2 Domain Phosphotyrosine Capture Kits – Thermo Scientific SH2 Domain Phosphotyrosine Capture Kits are fast and efficient systems for purifying active tyrosine phosphorylated proteins and interactors in mammalian cell lysates. Profile specific tyrosine phosphorylation events and identify SH2 domain protein-protein interaction networks.
• Monitor tyrosine phosphorylation events
• Profile receptor tyrosine-kinase activation
• Identify SH2 domain protein-protein interaction networks
• 17 unique SH2 domain capture kits available
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Apr 2009 |
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In-Cell ELISA Detection Kits – The Thermo Scientific In-Cell ELISA Colorimetric and Near Infrared Detection Kits are simple and convenient methods for quantification of
intracellular proteins levels in whole cells. The in-cell ELISA
is an accurate method to determine relative protein levels and degree
of post-translational modification among various cell types and
conditions.
• Near Infrared or Colorimetric Detection Kits Available
• Simple, fast assay format
• Higher throughput compared to Western blotting
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Mar 2009 |
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Subcellular Protein Fractionation Kit – The Thermo Scientific Subcellular Protein Fractionation Kit enables the stepwise separation and extraction of cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins from mammalian cultured cells or tissue.
• Fraction are compatible with a variety protein assays
• Obtain functional proteins for enzymatic and mobility shift assays
• Simple procedure uses no gradients or ultracentrifugation
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Mar 2009 |
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Pierce Rapid ELISA Mouse mAb Isotyping Kit – The Thermo Scientific Pierce Rapid ELISA Mouse mAb Isotyping Kit enables easy identification of mouse immunoglobulin class, subclass and light chain. This assay uses ELISA strip-plates pre-coated in different wells with anti-mouse heavy-chain capture antibodies or anti-mouse light-chain capture antibodies.
• Determine antibody isotype in ~1 hour
• Eight-well strip format allow partial use of plate
• Accurate characterization with samples containing >3ng/ml antibody
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Mar 2009 |
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Pierce Poly-HRP Conjugates and High Sensitivity HRP Conjugates – Thermo Scientific Poly-HRP and High Sensitivity HRP conjugates are horseradish peroxidase (HRP) conjugated goat anti-mouse, goat anti-rabbit or streptavidin and NeutrAvidin proteins that meet the demands for utmost sensitivity in ELISA, Western blotting and immunohistochemistry applications.
• Pre-diluted 10 µg/ml and 1 mg/ml stocks available
• Convenient stabilized liquid format
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Mar 2009
Dec 2008
Nov 2008 |
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Pierce Fast Western Blot Kit, ECL Substrate– The Thermo Scientific Pierce Fast Western Blot Kit with ECL Substrate contains optimized reagents that shorten the time to perform a typical Western blot from 4 hours down to approximately 55 minutes. No vacuum or special equipment is required.
• All optimized reagents included
• Kits for 5 or 25 blots (8 x 10 cm) available
• Requires only mouse or rabbit primary antibodies for target protein
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Mar 2009 |
