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Sodium Cholate is water soluble ionic detergent/bile salt commonly used in applications ranging from cell lysis, liposome preparation, isolation of membrane proteins and lipids, preventing nonspecific binding in affinity chromatography and as a cell culture media supplement.
Properties of Sodium Cholate
| Alternative Names: |
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(Cholate, Sodium Salt)
(Cholic Acid, Sodium Salt.)
(3 ,7 ,12 -Trihydroxy-5β-cholan-24-oic acid, monosodium salt) |
| Molecular Weight (MW): |
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430.6 |
| Aggregation Number: |
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2-4 (average) |
| Micelle MW: |
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900 (average) |
| Critical Micelle Concentration (CMC): |
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CMC (H2O) ~ 9-14 mM or ~ 3.875 - 6.028% (w/v) |
| Purity: |
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>99% by HPLC |
| Extinction Coefficient: |
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A280 1% detergent Solution: <0.08
A340nm: <0.04
A260nm: <0.1 |
| Storage: -20°C (internal) for long term storage, shipped ambient. |
- The effectiveness of a detergent in any application is dependent on the detergents concentration. Too much or too little detergent can often have a deleterious effect. It is recommended that researchers examine a variety of detergent concentrations in their application.
- At concentrations above 9.5 mM, cholate will form micelles having MW ~900-1200. The small size of these micelle allows them to be easily removed by dialysis or gel filtration if needed.
- Note: removal of a detergent from a protein solution my result in protein aggregation.
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Certificate of Analysis
Product Instructions
MSDS