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Concentrated Buffers  |
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Thermo Scientific Pierce Concentrated buffers save counter space and time.
Thermo Scientific Pierce Concentrated Buffers are ready to use without having to reconstitute with ultrapure water and, in the case of Tris-Glycine Buffer, methanol. Buffers are designed for use in dialysis, cross-linking, enzyme assays, ELISAs, immunohistochemistry, protein plate-coating, biotinylation and other applications. Keep our concentrated buffers close at hand to cover your lab’s research needs.
Highlights of Pierce Concentrated Buffers:
- Easy to use – no packets to open and no powder to dissolve
- Increased accuracy – eliminates the possibility of powder remaining in a packet
- Saves time – Concentration eliminates time spent waiting for powder to dissolve
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Buffer Type
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Application
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| 20X Borate Buffer |
Ideal for protein modification procedures requiring amine free buffer at alkaline pH. |
| 20X Tris-acetate-EDTA (TAE) Buffer |
Historically the most common buffer used for agarose gel electrophoresis in the analyses of nucleic acids. |
| 10X Tris-borate-EDTA (TBE) Buffer |
Often used for agarose gel electrophoresis in the analysis of nucleic acids. |
| 20X Modified Dulbecco’s PBS Buffer |
Used for wash buffers and antibody diluents in applications such as ELISA, Western blotting and other immunoassays. |
| 20X Modified Dulbecco’s PBS Tween-20 Buffer |
A wash buffer for ELISA, Western and other Immunoassays as well as a blocking buffer for plate based assays. |
| 20X Phosphate Buffered Saline Buffer |
Its ionic strength makes it ideal for crosslinking
and biotinylation requiring amine free buffer. |
| 20X Phosphate Buffered Saline Tween-20 Buffer |
A wash buffer for ELISA, Western and other Immunoassays as well as a blocking buffer for plate based assays. |
| 20X Tris Buffered Saline Buffer |
Used for wash buffers and antibody diluents in applications such as ELISA, Western blotting and other immunoassays. |
| 20X Tris Buffered Saline Tween-20 Buffer |
A wash buffer for ELISA, Western and other Immunoassays as well as a blocking buffer for plate based assays. |
| 10X Tris-Glycine Buffer |
Great for electrophoretic transfer from gel to membrane. |
| 10X Tris-Glycine SDS Buffer |
A running buffer for Tris-Glycine gel electrophoresis. |
20X Tris-HEPES SDS Buffer |
A running buffer for Tris-HEPES electrophoresis with Precise Precast Gels. |
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10X Pierce Western Blot Transfer Buffer
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A Western blot transfer buffer for transferring proteins from SDS-PAGE gels to nitrocellulose or PVDF membranes. This transfer buffer is compatible with tank transfer units and is specifically formulated to be used without methanol and without chilling. |
Related Products and Links:
BupH Dry Buffers– Convenient pouches ready to dissolve in water
Desalting and Buffer Exchange Columns and Plates
Dialysis Cassettes and Tubing
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Ordering Information
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 Certificate of Analysis  Product Instructions  MSDS |
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Product # |
Description |
Pkg. Size |
Files |
Price |
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Add
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28341 |
20X Borate Buffer
20X Concentrate, 1X = 50 mM Borate, pH 8.5 |
500 ml |
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$65.00 |
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Add
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28344 |
20X Modified Dulbecco's PBS Buffer
20X Concentrate, 1X = 8 mM Sodium Phosphate, 2 mM Potassium Phosphate, 0.14 M NaCl, 10 mM KCl, pH 7.4 |
500 ml |
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$65.00 |
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Add
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28346 |
20X Modified Dulbecco's PBS Tween-20 Buffer
20X Concentrate, 1X = 8 mM Sodium Phosphate, 2 mM Potassium Phosphate, 0.14 M NaCl, 10 mM KCl, 0.05% Tween-20, pH 7.4 |
500 ml |
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$65.00 |
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Add
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28348 |
20X Phosphate Buffered Saline
20X Concentrate, 1X = 0.01 M Sodium Phosphate, 0.15 M NaCl, pH 7.5 |
500 ml |
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$65.00 |
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Add
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28352 |
20X PBS Tween-20 Buffer
20X Concentrate, 1X = 0.01 M Sodium Phosphate, 0.15 M NaCl, 0.05% Tween-20, pH 7.5 |
500 ml |
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$65.00 |
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Add
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28354 |
20X TAE Buffer
20X Concentrate, 1X = 0.04 M Tris, 0.04 M Acetate, 0.001 M EDTA, pH 8.2-8.4 |
500 ml |
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$65.00 |
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Add
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28355 |
10X TBE Buffer
10X Concentrate, 1X = 0.089 M Tris, 0.089 M Borate, 0.002 M EDTA, pH 8.2-8.4 |
1L |
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$62.00 |
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Add
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28358 |
20X TBS Buffer
20X Concentrate, 1X = 25 mM Tris, 0.15 M NaCl, pH 7.2 |
500 ml |
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$65.00 |
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Add
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28360 |
20X TBS Tween-20 Buffer
20X Concentrate, 1X = 25 mM Tris, 0.15 M NaCl, 0.05% Tween-20, pH 7.5 |
500 ml |
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$65.00 |
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Add
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28362 |
10X Tris-Glycine SDS Buffer
10X Solution, 1X 0.025 M Tris, 0.192 M Glycine, 0.1% SDS, pH 8.5 |
1 L |
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$43.00 |
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Add
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28363 |
10X Tris-Glycine Buffer
10X Solution, 1X 0.025 M Tris, 0.192 M Glycine, pH 8.5 |
1 L |
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$43.00 |
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Add
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28368 |
20X Tris/HEPES/SDS Buffer
20X Concentrate, 1X = 0.1 M Tris, 0.1 M HEPES, 3 mM SDS, pH 8+0.25 |
500 ml |
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$65.00 |
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Add
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35040 |
10X Pierce Western Blot Transfer Buffer, Methanol-free
10X Concentrate, does not contain or require addition of methanol |
5 L |
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$88.00 |
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