Nonionic detergent for membrane protein extraction with may preserve protein activity better other detergents.

Thermo Scientific n-Dodecyl-β-D-Maltoside is especially useful for solubilizing membrane proteins to preserve their activity. The water soluble nonionic detergent is most often used for the isolation of hydrophobic membrane proteins. Multiple studies have shown that n-Dodecyl-β-D-maltoside is a gentle detergent that is often able to preserve protein activity better than many commonly used detergents, including NP-40, CHAPS and Octyl-β-glucoside.

Highlights of n-Dodecyl-beta-Maltoside:

• Lipid-like nonionic detergent
• Especially useful for isolating and stabilizing hydrophobic membrane proteins
• Preserves activity of membrane protein better than most of the detergents
• High-purity compound with low UV absorptivity

Properties of n-Dodecyl-β-D-Maltoside:

• Chemical Name: n-Dodecyl-beta-D-maltoside
• Molecular Weight: 510.6g
• Detergent Class: Nonionic
• Aggregation Number: 98 (average), 70 to 140 range
• Micelle Molecular Weight: 50,000g
• Critical Micelle Concentration (CMC): 0.17mM (0.009%, w/v) in water ; 0.12mM (0.006%, w/v) in 0.2M sodium chloride
• Cloud Point: Unknown
• Dialyzable: No

Product Details:

Like most detergents, n-Dodecyl-β-D-maltoside has dual hydrophobic/hydrophilic properties that facilitate lipid displacement and provide a lipid-like environment for membrane proteins. Studies suggest that the ability of these surfactants to preserve membrane protein structure stems in part from the reduced disruption of lipid:protein interactions where some of the natural lipid associations are maintained. While no detergent or set of conditions is optimal for all studies on membrane proteins, n-Dodecyl-β-D-maltoside has unique characteristics that have made it especially helpful in certain protein methods.

Specifications for n-Dodecyl-beta-maltoside (Part No. 89902, 89903):

• Formula: C₂₄H₄₆O₁₁
• Molecular Weight: 510.6g
• Purity (by HPLC): ≥99%
• Absorbance (1% Detergent Solution): 340nm <0.02; 280nm <0.04; 260nm <0.06; 225nm <0.1
• pH (1% Solution): 5 to 8
• Solubility (in water at 0 to 5°C): ≥20%
• Conductivity (10% Solution) : <40mS

References:

1. Casey, J. R.., et al. (1993). Biochemistry, 32, 1172-1179.
2. Engel, C.K., et. al. (2002). Biochimica et Biophysica Acta, 1564, 47-56.
3. Boulter, J. M., Wang, D. N., (2001). Protein Expression and Purification, 22, 337-348.
4. le Maire, M., et. al. (2000). Biochimica et Biophysica Acta, 1508, 86-111.
5. Banerjee, P., et. al. (1995). Chemistry and Physics of Lipids, 77, 65-78.
6. Wang, D. N., et. al. (2003). Biochimica et Biophysica Acta, 1610, 23-36.
7. Berger,B. W., et. al. (2005). Biophysical Journal, 89, 452-464.
8. Auer, M. et. al. (2001). Biochemistry, 40, 6628-6635.

Related Products and Resources:

Detergent Sampler Pack
Browse all Thermo Scientific Pierce Detergents
Protein Methods Library: Detergents for Cell Lysis