 |
n-Dodecyl-b-D-maltoside maltoside is a water soluble nonionic detergent often used in the isolation of membrane proteins. Multiple studies have shown that n-Dodecyl-b-D-maltoside is a gentle detergent that is often able to preserve protein activity better than many commonly used detergents, including Triton x-100, NP-40,CHAPs and Octyl-b-glucoside (1-8).
Like most detergents, n-Dodecyl-b-D-maltoside has dual hydrophobic/hydrophilic properties that allow lipid displacement and provides a lipid-like environment for membrane proteins, however, studies suggest the ability to preserve membrane protein structure by n-Dodecyl-b-D-maltoside, and other gentle detergents, in part stems from reduced disruption of lipid:protein interactions where some of the natural lipid associations are maintained. While no detergent or set of conditions is optimal for all studies on membrane proteins; n-Dodecyl-b-D-maltoside has been shown to be a versatile and reliable detergent.
Properties of n-Dodecyl-b-D-maltoside
| Molecular Weight (MW): |
|
510.6 |
| Aggregation Number: |
|
98 (average) 70-140 range. |
| Micelle MW: |
|
50,000 (average) |
| Critical Micelle Concentration (CMC): |
|
CMC (H2O) ~ 0.17 mM or ~ 0.009% (w/v).
CMC (0.2 M NaCl) ~ 0.12mM or ~ 0.006% (w/v). |
| Purity: |
|
>99% by HPLC |
| Extinction Coefficient: |
|
A280 1% detergent Solution: <0.04
A340nm: <0.02
A260nm: <0.06 |
| Storage: -20°C (internal) for long term storage, shipped ambient. |
At concentrations above 0.17 mM n-Dodecyl-b-D-maltoside micelles having MW >50,000. In this form the detergent is difficult to remove by dialysis or gel filtration. Ionic strength of the solution has minimal effect on the CMC.
References
- Casey, J. R.., et al. (1993) Detergent Interaction with Band 3, a Model Polytopic Membrane Protein. Biochemistry, 32, 1172-1179.
- Engel, C.K., et. al. (2002) Stability of lactose permease in detergent solutions. Biochimica et Biophysica Acta, 1564, 47-56.
- Boulter, J. M., Wang, D. N., (2001) Purification and Characterization of Human Erythrocyte Glucose Transporter in Decylmaltoside Detergent Solution. Protein Expression and Purification, 22, 337-348.
- le Maire, M., et. al. (2000) Interaction of membrane proteins and lipids with solubilizing detergents. Biochimica et Biophysica Acta, 1508, 86-111.
- Banerjee, P., et. al. (1995) Differential solubilization of lipids along with membrane proteins by different classes of detergents. Chemistry and Physics of Lipids, 77, 65-78.
- Wang, D. N., et. al. (2003) Practical aspects of overexpressing bacterial secondary membrane transporters for structural studies. Biochimica et Biophysica Acta, 1610, 23-36.
- Berger,B. W., et. al. (2005) Relating Surfactant Properties to Activity and Solubilization of the Human Adenosine A3 Receptor. Biophysical Journal, 89, 452-464.
- Auer, M. et. al. (2001) High-Yield Expression and Functional Analysis of Escherichia coli Glycerol-3-phosphate Transporter. Biochemistry, 40, 6628-6635.
|
|
Certificate of Analysis
Product Instructions
MSDS