Endogen's TriSpot Human IFNγ/IL-2 ELISPOT Kit is a tri-color enzyme-linked immunospot (ELISPOT) assay for the quantitation of single cells releasing both human interferon gamma (IFNg) and interleukin-2 (IL-2). A spot forms at the site where secreted cytokine is bound, is counted (either using a dissection microscope or automated ELISPOT reader) and then compared to the number of cells added to the microplate. This allows the frequency of cells secreting each cytokine to be calculated. One spot is equal to one molecule-secreting cell. IFNγ secretion produces blue spots, IL-2 spots are red and dual secretion is purple.

• Species: Human
• Analyte:   IFN gamma/IL-2
• Format:  96-well PVDF membrane bottom plate 
• Total Assay Time:  3 hours
• Sample Size:  50 µl/well

A dual color enzyme-linked immunospot (ELISPOT) assay for the quantitation of single cells releasing both human interferon gamma (IFNγ) and interleukin-2 (IL-2).

  Features and Benefits:

• Analyze both IFN gamma and IL-2 in one well - both analytes are analyzed at the same time in each well of an ELISPOT plate
• Consistent Membrane Coating - stringent quality control procedures ensure well-to-well and within-well consistency
• Optimal Plate Blocking - an optimized blocking procedure increases signal-to-noise ratios and lowers background values
• Well-Characterized Antibody Pairs - specific, high-affinity antibody pairs provide optimal sensitivity
• Ease-of-Use - pre-coated plates, pre-titered reagents, and a simple assay procedure make testing simple
• Short Assay Time - development of spots is completed in just 2½ hours following cell culture, offering fast, accurate results

Analysis of both IFNγ and IL-2 in each well of an ELISPOT plate

PHA stimulated PBMCs evaluated using Endogen’s TriSpot™ Human IFNγ/IL-2 ELISPOT Kit

Instrumentation Options:
For more information on our instruments for reading ELISAs, visit www.thermo.com/readingroom