Krypton Protein Stain
A fast, affordable fluorescent stain that provides excellent performance

Krypton Protein Stain is a fluorescent stain for detecting proteins in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and 2-D gels. The stain’s unique formulation reduces the time and cost associated with typical fluorescent staining (Figures 1-2, Table 1). Krypton Protein Stain is a decisive improvement in fluorescent stain technology, providing sensitivity equivalent to, or greater than, other fluorescent stains, while minimizing protein quantitation problems associated with differential protein staining (Figures 3-4). The stain provides high signal intensity with a linear quantitative range of three to four orders of magnitude across a broad range of protein types, which maximizes the detection of low-abundant proteins (Figures 4-5).

Features		Benefits
	Fast		Detect as little as 2 ng protein/band in just 30 minutes (using rapid protocol)
	Detection limit: < 0.25 ng protein/band		Maximizes the detection of low-abundant proteins
	Minimal differential staining of proteins		Linear response to staining for proteins of various sizes and containing post-translational modifications
	Excitation/Emission maxima: 520/580 nm		Compatible with commonly available instrumentation and filter sets
	Versatile		Compatible with mass spec analysis and protein sequencing
	Linear quantitative range of 3-4 orders of magnitude		Accurately and reproducibly visualize proteins over a broad range of concentrations

Figure 1. Krypton Protein Stain is fast and sensitive. Proteins were separated in 4-20% Tris-glycine gels and stained using the indicated protocols. The gels were imaged with the Typhoon* 9410 at 532 nm excitation and 580 BP30 emission.

Figure 2. Krypton Protein Stain works up to five times faster than other fluorescent stain protocols.

Figure 3. Krypton Protein Stain provides sensitivity equal to or greater than other fluorescent stains in 2-D gel analysis. Processed HeLa cell protein extract (28 µg) was focused on a pH 5-8 IPG strip followed by 4-20% SDS-PAGE. The gels were stained and imaged following manufacturer-recommended basic protocols.

Figure 4. Krypton Protein Stain produces a linear response to staining with minimal protein-to-protein variation. Relative fluorescent intensity was plotted as a function of protein quantity for proteins of various sizes and containing post-translational modifications.

Figure 5. Krypton Protein Stain exhibits a more linear quantitative range than other fluorescent stains. Relative fluorescent intensity for each protein concentration is the average of nine different proteins separated in 4-20% Tris-glycine and Precise Gels. Error bars represent the standard deviation for triplicate gels. The gels were stained and imaged according to the manufacturer’s basic protocols.

U.S. Patent Pending on Krypton Protein Stain Technology.