Save yourself from the dreaded silver stain "do-over!"

New Pierce Silver Stain Rescue Reagent salvages
a less-than-perfect gel for display or publication

Overdeveloped Silver Stained Gel
Overdeveloped Silver-stained Gel	Gel Treated with Silver Stain Rescue Reagent	Properly Developed   Control Gel
Figure 1. High background removed by the Silver Stain Rescue Reagent. Dilutions of Escherichia coli cell lysate were separated by sodium dodecyl sulfate-polyacrylamide gel eletrophoresis (SDS-PAGE) using 4-12% gradient gels and stained with SilverSNAP Stain Kit II (Product # 24612). Rescue Reagent working solution for high background (0.2 ml reagent A, 0.2 ml Reagent B in 50 ml of ultrapure water) was used. Stain removal was terminated after 11 minutes.

Unevenly Developed Silver Stained Gel
Unevenly developed Silver-stained Gel	Gel Treated with Silver Stain Rescue Reagnet	Properly Developed   Control Gel
Figure 2. Uneven background stain removed by the Silver Stain Rescue Reagent. Dilutions of an unstained protein molecular weight marker were separated by SDS-PAGE using 4-12% gradient gels and stained with SilverSNAP Stain Kit II (Product No.24612). Rescue Reagent working solution for low/non-uniform background (0.1 ml reagent A, 0.1 ml Reagent B in 50 ml of ultrapure water) was used. Stain removal was terminated after 55 minutes.

• Easy-to-use - mix the reagents at the recommended dilution and you're ready to rescue a gel with an overdeveloped or non-uniform background
• Fast acting - attenuates background in minutes, allowing you to remove the appropriate amount of background to meet your objective; complete the entire process in ~1 hour
• Economical and efficient - costs just $0.50-$0.99 per mini gel, coupled with a short process time
• Preserves data - removes silver from the gel uniformly, enabling improved band visibility without altering the data
• Compatible with any commercial or homemade silver stain - removes high background or non-uniform staining, regardless of the silver stain formulation

Protocol