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YOU ARE HERE:  Browse Product Catalog > Protein Electrophoresis and Staining > Staining Kits and Reagents > Metal Based Stains > Silver Stain Rescue Reagent  


Silver Stain Rescue Reagent 

Save yourself from the dreaded silver stain "do-over!"

New Pierce Silver Stain Rescue Reagent salvages
a less-than-perfect gel for display or publication

Have you ever been frustrated by a silver-stained gel that didn't turn out just right for that big meeting or publication deadline? The new Silver Stain Rescue Reagent from Pierce can recover an out-of-control gel, while saving you the time and frustration of having to start over reloading the sample, running the gel and repeating the silver staining protocol. With Silver Stain Rescue Reagent, most mini gels are rescued in less than one hour, depending on the working concentration selected.

Overdeveloped Silver Stained Gel
Overdeveloped Silver-stained Gel Gel Treated with Silver Stain Rescue Reagent   Properly Developed
  Control Gel

Figure 1. High background removed by the Silver Stain Rescue Reagent. Dilutions of Escherichia coli cell lysate were separated by sodium dodecyl sulfate-polyacrylamide gel eletrophoresis (SDS-PAGE) using 4-12% gradient gels and stained with SilverSNAP Stain Kit II (Product # 24612). Rescue Reagent working solution for high background (0.2 ml reagent A, 0.2 ml Reagent B in 50 ml of ultrapure water) was used. Stain removal was terminated after 11 minutes.



Unevenly Developed Silver Stained Gel
Unevenly developed Silver-stained Gel Gel Treated with Silver Stain Rescue Reagnet   Properly Developed
  Control Gel

Figure 2. Uneven background stain removed by the Silver Stain Rescue Reagent. Dilutions of an unstained protein molecular weight marker were separated by SDS-PAGE using 4-12% gradient gels and stained with SilverSNAP Stain Kit II (Product No.24612). Rescue Reagent working solution for low/non-uniform background (0.1 ml reagent A, 0.1 ml Reagent B in 50 ml of ultrapure water) was used. Stain removal was terminated after 55 minutes.

Highlights:
  • Easy-to-use - mix the reagents at the recommended dilution and you're ready to rescue a gel with an overdeveloped or non-uniform background
  • Fast acting - attenuates background in minutes, allowing you to remove the appropriate amount of background to meet your objective; complete the entire process in ~1 hour
  • Economical and efficient - costs just $0.50-$0.99 per mini gel, coupled with a short process time
  • Preserves data - removes silver from the gel uniformly, enabling improved band visibility without altering the data
  • Compatible with any commercial or homemade silver stain - removes high background or non-uniform staining, regardless of the silver stain formulation
Protocol


Ordering Information
Certificate of AnalysisCertificate of Analysis   Product InstructionsProduct Instructions   MSDSMSDS
Buy Product # Description Pkg. Size Files Price
Add 24614 Silver Stain Rescue Reagent
Sufficient material to rescue the results from 100-200 minigels
40 ml Product Instructions MSDS for product # 24614 Silver Stain Rescue Reagent $112.00

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