Protein-Free Blocking Buffers

 Problem: Traditional blocking buffers contain proteins that can cross-react with your system, resulting in high background and reduced signal (Figure 1-2).

Solution: Use Protein-Free Blocking Buffers to eliminate or minimize cross-reactivity associated with protein-based blocking buffers in ELISA, Western blotting, arrays and other immunodetection applications.

Features

Benefits

Eliminate or minimize cross-reactivity associated with protein-based blocking buffers

Can be used in Western blots, ELISA or arrays
Does not interfere with avidin-biotin systems

     High signal-to-background

Optimal sensitivity

     1X formulation

Ready-to-use

     Available with 0.05 %
       Tween-20 already added

Save time and money

Figure 1. Protein-Free Blocking Buffers exhibit less background than other blocking buffers in a multiplex ELISA array.  "SearchLight Arrays" were created by spotting up to 12 cytokine capture antibodies per well. The plates were then blocked with the indicated blocking buffers and the background for each well determined. Error bars represent the standard deviation for triplicate microplate wells.

Figure 2. Protein-Free Blocking Buffer efficiently blocks Western Blotting membranes.
Jurkat Apoptotic Lysate (lane 1: 0.25 µg, lane 2: 0.50 µg) was separated in 4-20% Tris-glycine gels and transferred to nitrocellulose or PVDF membranes. The membranes were blocked for 1 hour at room temperature with the indicated blocking buffer, probed with mouse anti-PARP (0.25ug/ml) followed by goat anti-mouse HRP (4ng/ml) and detected by chemiluminescence with SuperSignal West Dura Substrate.

Related Products
Protein-based Blocking Buffers
Pierce ECL Western Blotting Substrate
Restore Western Blot Stripping Buffer
Additional Pierce Blocking Buffers
SuperSignal West Pico Chemiluminescent Substrate
SuperSignal ELISA Pico Chemiluminescent Substrate
1-Step Ultra TMB

Technical Resources
Western Blotting Technical Handbook
ELISA Technical Handbook