Successfully block Western blots and ELISA plates without introducing any exogenous proteins that might cross-react with assay components.

Thermo Scientific Pierce Protein-Free Blocking Buffers are ready-to-use TBS and PBS formulations of a blocking agent that is devoid of any protein components while being highly effective at blocking protein binding surfaces for Western blotting, ELISA and other methods.

Problem: Traditional blocking buffers contain proteins that can cross-react with your system, resulting in high background and reduced signal.

Solution: Use Protein-Free Blocking Buffers to eliminate or minimize cross-reactivity associated with protein-based blocking buffers in ELISA, Western blotting, arrays and other immunodetection applications.

Highlights:

• Protein-Free – eliminate or minimize cross-reactivity associated with protein-based blocking buffers
• Compatible – effective in all kinds of protein detection systems, including Western blots (membranes), ELISA (microplates) and arrays (coated glass slides); does not interfere with avidin-biotin systems
• High-performance – optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)
• Convenient – supplied as ready-to-use 1X TBS and PBS formulations with and without 0.05% Tween*-20

Protein-Free Blocking Buffers exhibit less background than other blocking buffers in a multiplex ELISA array. SearchLight" microplate protein arrays were created by spotting up to 12 cytokine capture antibodies per well. The plates were then blocked with the indicated blocking buffers and the background for each well determined. Error bars represent the standard deviation for triplicate microplate wells.

Protein-Free Blocking Buffer efficiently blocks Western Blotting membranes. Jurkat Apoptotic Lysate (lane 1: 0.25µg, lane 2: 0.50µg) was separated in 4-20% Tris-glycine gels and transferred to nitrocellulose or PVDF membranes. The membranes were blocked for 1 hour at room temperature with the indicated blocking buffer, probed with mouse anti-PARP (0.25ug/mL) followed by goat anti-mouse HRP (4ng/mL) and detected by chemiluminescence with Thermo Scientific SuperSignal West Dura Substrate.

Related Products:

Pierce ECL Western Blotting Substrate
Restore Western Blot Stripping Buffers
Additional Pierce Blocking Buffers
SuperSignal West Pico Chemiluminescent Substrate
SuperSignal ELISA Pico Chemiluminescent Substrate
1-Step Ultra TMB

Additional Resources:

Western Blotting Technical Handbook
ELISA Technical Handbook