Spin columns to efficiently handle small volumes of resin for immunoprecipitation and affinity purification!

Thermo Scientific Pierce Spin Cups and Spin Columns are convenient tools for manipulating small volumes of affinity supports (5–500 µl) for protein purification. Simply add the affinity resin and sample to one of the affinity columns, then use a microcentrifuge to efficiently wash away contaminants and elute your purified sample without losing any resin in the process. Spin columns allow you to affinity purify more protein in less time!

Applications for Spin Columns

• Affinity purification / Affinity chromatography
• Immunodepletion
• Immunoprecipitation (IP)
• Co-immunoprecipitation (co-IP)

Spin Column Highlights for IP and Co-IP

• Efficient washing of samples means fewer washes are needed to remove contaminating proteins
• Efficient elution of samples means more antigen and co-precipitated proteins are recovered
• No resin loss means more consistent IP and co-IP results
• Decanting supernatant from IP or co-IP pellet is not necessary
• Spin protocols drastically reduce the time required for IP’s and co-IP’s
• Low protein binding polypropylene column construction minimizes nonspecific binding

Pierce Spin Cups – Paper Filter with collection tubes
View Dimensions of Spin Cup – Paper Filter (Opens a new browser window)	Paper filters are resistant to clogging from cellular debris. Column volume: 600 µl Resin volume: 20-300 µl Filter type: Paper filter, ~10 µm pore size Cap: Collection tube cap fits onto inserted spin cup
Pierce Spin Cups – Cellulose Acetate Filter with collection tubes
View Dimensions of Spin Cup – Cellulose Acetate Filter (Opens a new browser window)	Smaller pore size than paper. Column volume: 800 µl Resin volume: 20-400 µl Filter type: Cellulose acetate filter, 0.45 µm pore size Cap: Collection tube cap fits onto inserted spin cup
Pierce Spin Columns – Screw Cap with Luer-Lok adaptors
View Dimensions of Spin Column with Screw Caps (Opens a new browser window)	Luer-Lok adaptors allow these columns to be used for syringe-based purifications. Column volume: 900 µl Resin volume: 20-400 µl Filter type: Polyethylene filter, ~10 µm pore size Small & large frit options for different sample sizes Cap: O-ring screw top caps Press-in bottom plugs
Pierce Spin Columns – Snap Cap with collection tubes
View Dimensions of Spin Column with Snap Caps (Opens a new browser window)	These are the spin columns used in the Cell Surface Protein Isolation and Glycoprotein Isolation Kits. Column volume: 1000 µl Resin volume: 20-500 µl Filter type / pore size: Polyethylene filter, ~30 µm pore size Cap Snap cap on column (no cap on collection tube) Press-on bottom caps
Pierce Micro-Spin Columns
View Dimensions of Micro-Spin Column (Opens a new browser window)	Column volume: 400 µl Resin volume: 5-100 µl Filter type: Polyethylene filter, ~30 µm pore size Cap: O-ring screw top caps Press-on bottom caps

Related Products
Larger spin columns for affinity purification
Gravity-flow columns for affinity purification
Affinity purification supports
Immunoprecipitation kits

References
Anzai, N., et al. (2004) J. Biol. Chem.279, 45942-45950.
Sokolov, M., et al. (2004) J. Biol. Chem.279, 19149-19156.
Wu, X., et al. (2005) J. Biol. Chem.280, 29964-29970.
Lee, J-S. et al. (2006) J. Virol.80, 4079-4087.