Compatible with LI-COR Odyssey and other infrared imaging systems

Krypton* Infrared Protein Stain† is a fluorescent stain for detecting proteins in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and 2-D gels. Researchers now have an easy-to-use, high-performance fluorescent protein stain for the near-infrared region of the spectrum compatible with the LI-COR Odyssey* Infrared Imaging System and other commonly available CCD instruments (Figure 1). The stain delivers substantial improvements in protein-staining performance compared to coomassie stains (Figure 2). Krypton Infrared Protein Stain exhibits minimal protein-to-protein variation and provides high signal intensity with a linear response to staining (Figure 3-4).

Highlights:

• Excitation/emission maxima – 690/718 nm
• Instrument-compatible – ideal for LI-COR Odyssey Instruments and other CCD instrumentation
• Versatile – compatible with mass spectrometry
• Wide quantitative range – three to four orders of magnitude
• Sensitive – 0.25 ng protein can be detected with the basic protocol (~2 hours)
• Fast – detect down to 2 ng protein with the rapid protocol (~1 hour)

Figure 1. Krypton Infrared Protein Stain protocol.

Figure 2. Krypton Infrared Protein Stain is up to 130 times more sensitive than coomassie stain. Proteins were separated in 4-20% Tris-glycine gels and stained with Krypton Infrared Protein Stain or coomassie stain (GelCode* Blue Stain Reagent, Product # 24592). The gels were imaged with the Odyssey Infrared Imaging System at 680nm excitation and 720 nm emission.

Figure 3. Krypton Infrared Protein Stain produces a linear response to staining with minimal protein-to-protein variation. Relative fluorescent intensity was plotted as a function of protein quantity for proteins of various sizes and containing post-translational modifications. The relative fluorescent intensity for each data point is the average value for triplicate gels.

Figure 4. Krypton Infrared Protein Stain provides exceptional sensitivity and low background in 2-D analysis. Processed HeLa cell protein extract (28 µg) was focused on a pH 5-8 IPG strip followed by 4-20% SDS-PAGE. The gel was stained using the basic protocol (~2 hours) and imaged with the Odyssey Infrared Imaging System using the 700 nm channel.

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Ordering Information

Bulk quantities: Inquire    Certificate of Analysis   Product Instructions   MSDS

Product # Description Pkg. Size Files Price 53070 Krypton Infrared Protein Stain (10X) Sufficient reagent to stain 4 mini gels (8 x 10 cm) 20 ml $40.00 53071 Krypton Infrared Protein Stain (10X) Sufficient reagent to stain 20 mini gels (8 x 10 cm) or two to four large-format gels 100 ml $114.00 53072 Krypton Infrared Protein Stain (10X) Sufficient reagent to stain 100 mini gels (8 x 10 cm) or 10 to 20 large-format gels. 500 ml $412.00