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 Few technical manuals have become standards in bioresearch. Antibodies: A Laboratory Manual by Ed Harlow and David Lane has enjoyed that status for more than a decade. The authors, however, have raised the standard with the publication of their new book, Using Antibodies: A Laboratory Manual.
Harlow and Lane have completely revised their guide to the use of antibody reagents in the laboratory. Chapters have been entirely rewritten, reorganized and updated to provide background, context and step-by-step instructions for techniques ranging from choosing the right antibody and handling it correctly, to the proper methods for characterizing antigens in cells and solutions. They’ve also added new chapters on tagging proteins and epitope mapping.
Rather than presenting an array of solutions for working with antibodies and antigens, Using Antibodies identifies the best approach to specific problems. These recommendations include more detail in the protocols, extensive advice on avoiding and solving problems, information regarding proper controls, and extensive illustration of theory, methods and results. The book also includes a bonus – a set of portable protocols that include step-by-step instructions for the most frequently used and essential techniques. The protocols are printed on durable cards, enabling them to be used easily at the bench.
This helpful guide, along with high-quality products from Pierce, will help you purify, immobilize, label and store antibodies and perform common procedures such as immunoprecipitation, Western blotting and ELISA.
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