Affinity and desalting resins in cartridges for liquid chromatography (LC).

The Thermo Scientific Pierce Chromatography Cartridges are convenient, reliable, ready-to-use, pre-packed 1-ml and 5-ml columns of sample-prep and affinity-purification resins for manual or automated liquid chromatography (LC).

The cartridge-fittings are compatible with the popular automated liquid-chromatography systems or for manual syringe processing. The cartridges attach directly to ÄKTA* or FPLC Systems without additional connectors. Cartridges can be used individually or connected in a series to obtain even higher column capacity. Each product supplied in the Pierce Chromatography Cartridge format includes an accessory pack that readily adapts cartridges for use with Luer-Lok* Syringe Fittings or tubing. The cartridges provide fast, easy and reproducible chromatographic separations and can be regenerated for multiple uses.

Chromatography Cartridge Highlights:

• Two sizes – 1 ml and 5 ml, convenient for typical research scales
• Compatible – fittings allow connection with popular LC systems or a standard syringe
• Versatile – use singly or connected in series to service different capacity requirements
• Validated – available cartridges have been tested to ensure performance in the format
• Reusable – accessory pack includes caps for convenient storage between uses
• Economical – comparable performance at lower cost than other commercially available cartridges

Performance Data:

Efficient salt removal and protein recovery with Desalting Chromatography Cartridge. Bovine serum albumin (1 mg) in 1 M NaCl was applied to 5-ml Thermo Scientific Zeba Desalting Cartridge at a flow rate of 5 ml/minute. Cartridge profile shows isocratic elution of BSA (blue) and NaCl detected by conductivity (brown). Greater than 95% of the BSA was recovered and more than 95% of the was salt removed. Results were comparable to those obtained with desalting cartridges from other suppliers (see Note).

Excellent antibody yield and purity acheived with Protein A Chromatography Cartridge. Normal human serum (60 mg) was applied in PBS to a 1-ml Thermo Scientific Pierce Protein A Cartridge and eluted with 0.1 M glycine, pH 2.8, using a flow rate of 1 ml/minute. The arrow denotes the start of the low pH elution. The yield of human IgG was 6.85 mg. Fractions were separated by SDS-PAGE and the gel was stained with Imperial* Protein Stain (Product # 24615). M = MW marker, L = sample load, FT = flow-through and E = elution. Results were comparable to those obtained with a GE Healthcare HiTrap* Column (see Note).

Note: see additional Performance Data for Pierce Chromatography Cartridges, including side-by-side comparison to more expensive columns from other suppliers.

Available Pierce Chromatography Cartridge Products:

• Desalting Resins:
  • Desalting Cartridges (contain Zeba Desalting Resin)
• Antibody Purification Resins:
  • Protein A Cartridges (contain Pierce Protein A Agarose)
  • Protein G Cartridges (contain Pierce Protein G Agarose)
  • Protein L Cartridges (contain Pierce Protein L Agarose)
  • Protein A/G Cartridges (contain Pierce Protein A/G Agarose)
  • Melon Gel Cartridges (contain Melon Gel Resin)
• Biotin Binding Resins:
  • High Capacity Streptavidin Cartridges (contain Streptavidin Agarose)
  • High Capacity NeutrAvidin Cartridges (contain NeutrAvidin Agarose)
• Cobalt (IMAC) Resins:
  • HisPur Cartridges (contain HisPur Cobalt Agarose Resin)
• Phosphoprotein Purification:
  • Pierce Phosphoprotein Enrichment Cartridges

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