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Protein Stabilizing Cocktail  |
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Don't stress out your protein!
Our Protein Stabilizing Cocktail extends shelf-life of precious proteins
The Protein Stabilizing Cocktail is a versatile stabilizing solution that increases the shelf-life of purified or partially purified proteins during routine storage. The proprietary formulation of low-molecular weight, naturally occurring molecules helps protect proteins from environmental stresses that can otherwise lead to enzyme inactivation, aggregation and freeze-thaw damage.
The Protein Stabilizing Cocktail is provided as an easily pipettable, buffered 4X concentrate. After dilution in Protein Stabilizing Cocktail, the protein may be stored in the manner typical for the specific protein (4ºC or -20ºC). Although the degree of stabilization is protein-specific, the cocktail significantly stabilizes most proteins compared with conventional buffer alone. The Protein Stabilizing Cocktail is nontoxic and does not destabilize biomolecules; however, all cocktail components can be removed by dialysis or desalting before use in downstream assays, if desired.
Highlights:
- Stabilizes most proteins over buffer alone
- Does not destabilize biomolecules in downstream assays
- All components are dialyzable
- Easy to pipette (vs. 50% glycerol)
Proteins Classes Tested:
- Kinase
- Phosphatase
- Peroxidase
- Restriction Enzyme
- Luciferase
- Cytokine
- Antibody
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| Figure 1. Luciferase activity stabilized by Protein Stabilizing Cocktail. Commercially available luciferase was stored at 50 µg/ml in Protein Stabilizing Cocktail from Pierce in a 30°C accelerated stability study. Fluorescence was measured at time 0 (dark bars) and after 1, 2, 4 and 6 weeks of storage. Luciferase stored in Protein Stabilizing Cocktail maintained 85% of its original activity after four weeks compared to a Tris-buffered salt formulation (control) and competitors' stabilizing agents, which were completely inactive after two weeks. |
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Certificate of Analysis
Product Instructions
MSDS