Fast and effective protein desalting for tiny and large volume samples (2µL to 4mL) with any standard benchtop centrifuge.

Thermo Scientific Zeba Spin Desalting Columns, 7K MWCO, are polypropylene centrifuge columns containing high-performance Zeba Desalting Resin, which offers rapid and exceptional protein desalting with high recovery. Available in 5 different columns sizes, Zeba Spin Desalting Columns provide trouble-free desalting and buffer exchange for sample volumes ranging from 2µL to 4mL.

The easy-to-use spin column format dramatically improves results over standard drip-column methodologies, eliminating the need to wait for samples to emerge by gravity flow and the need to monitor fractions for protein recovery. Zeba Desalting Columns require no chromatographic system, cumbersome column preparation or equilibration, and allow multiple samples to be processed in less than ten minutes.

Highlights of Zeba Spin Desalting Columns, 7K MWCO:

• Exceptional protein recovery
• No screening fractions for protein or waiting for protein to emerge by gravity-flow
• Wide product offering accommodates samples 2µL to 4mL
• Easy-to-use with no cumbersome column preparation or equilibration
• Minimal sample dilution

About Zeba Desalting Spin Columns

Zeba Spin Desalting Columns contain a proprietary high-performance desalting resin that offers exceptional desalting and protein-recovery characteristics compared to other commercially available resins. Even very dilute (25µg/mL) protein samples can be successfully processed to obtain greater than 95% retention (removal) of salts and other small molecules (< 1,000 MW) and good recovery of proteins and other macromolecules (> 7,000 MW).

Despite claims, other commercially available resins perform only satisfactorily in spin columns with sample of high concentration (>250µg/mL) over a narrow window of sample volumes. The proprietary Zeba Micro Spin Desalting Columns has exceptional protein recovery and desalting characteristics with >95% retention of salts and other small molecules of <1,000 MW and are recommended for processing compounds >7,000 MW.

Effect of sample volume on protein recovery. Samples of bovine serum albumin at 1mg/mL were desalted with a Zeba Micro Desalting Column. Recovery percentages were analyzed by BCA Protein Assay (Product # 23225). Sample volumes were 3µL or 10µL with a 3µL buffer stacker placed over the sample.

Related Products:
Zeba Spin Desalting Columns, 40K MWCO
Zeba 96-well Spin Desalting Plates (sample volumes 20 to 100µL)
Zeba Desalting Chromatography Cartridges (sample volumes 50µL to 1.5mL)
Pierce Centrifuge Columns (empty)
Desalting Product Selection Guide– Compare all Thermo Scientific Pierce desalting products