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Biotin Labeling of Antibodies and Other Proteins  |
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Specifically label primary amines (lysine and N-terminus) with biotin, the most common reactive groups found on the surface of proteins.
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Specifically biotinylate carbohydrate modifications on glycoproteins. This method is advantageous for labeling antibodies because the biotin is attached to the Fc region and immunoreactivity is not comprimised.
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Use EDC cross-linking to attach these amine-containing biotin labeling reagents to carboxyl groups found on aspartic- and glutamic-acid residues and at the c-terminus.
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Photoreactive and other specialty reagents help you biotin label proteins, nucleic acids or organic molecules that don't have a readily available functional group.
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Specifically attaching a biotin label to sulfhydryl groups can aid in preserving the biological activity of many proteins.
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The Pierce Cell Surface Protein Isolation Kit provides a simple and efficient method to specifically label and purify cell surface proteins for western blot analysis.
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Simple and accurate methods to determine the amount of biotin attached to proteins or other biomolecules following biotinylation.
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