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Sodium Deoxycholate is water soluble ionic detergent/bile salt commonly used in applications ranging from cell lysis (RIPA Buffer), liposome preparation, isolation of membrane proteins and lipids, preventing nonspecific binding in affinity chromatography and a cell culture media supplement.
Sodium Deoxycholate is the detergent recommended for stripping endotoxin (Lipopolysaccharide or LPS) from our Detoxi-Gel Endotoxin Removing Gel. Scientists using Detoxi-Gel Endotoxin Removing Gel will also use this detergent.
Properties of Sodium Deoxycholate
| Alternative Names: |
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(Deoxycholate, Sodium Salt)
(3 ,12 a-Dihydroxy-5β-cholan-24-oic acid, mono sodium salt) |
| Molecular Weight (MW): |
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414.6 |
| Aggregation Number: |
|
5 (average) |
| Micelle MW: |
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2000 (average) |
| Critical Micelle Concentration (CMC): |
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CMC (H2O) ~ 2-6 mM or ~0.083 - 0.249% (w/v) |
| Purity: |
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>98% by HPLC |
| Solubility in water: |
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>5% |
| Extinction Coefficient: |
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A280 1% detergent Solution: <0.04
A340nm: <0.02
A260nm: <0.06 |
| Storage: -20°C (internal) for long term storage, shipped ambient. |
- The effectiveness of a detergent in any application is dependent on the detergents concentration. Too much or too little detergent can often have a deleterious effect. It is recommended that you examine a variety of detergent concentrations in your application.
- At concentrations above 2 mM, cholate will form micelles having MW ~2000. The small size of these micelle allows them to be easily remove by dialysis or gel filtration if needed.
- Note: removal of a detergent from a protein solution my result in protein precipitation and/or aggregation.
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Certificate of Analysis
Product Instructions
MSDS