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Cell Lysis and Protein Extraction  |
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Gentle whole cell lysis buffers for high-yield protein extraction and solubilization from E. coli and other bacteria, cultured mammalian cells and tissues, insect cells, yeast cultures, and flowering plants.
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Optimized reagent kits for subcellular fractionation of proteins and isolation of organelles such as nuclei, mitochondria, peroxisomes and lysosomes by Dounce-homogenization or density-gradient separation.
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Reagent-based kits optimized for stepwise separation, enrichment and extraction of proteins from different cell fractions; isolate cytoplasmic, membrane, nuclear, chromatin-bound and cytoskeletal proteins for downstream analysis.
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Ready-to-use, inhibitor cocktails (not tablets) and individual purified formulations of protease inhibitors and phosphatase inhibitors to protect proteins from degradation during cell lysis and extraction.
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Formulations of enzymes such as lysozyme, deoxyribonuclease I (DNase I) and micrococcal nuclease (MNase) to aid and facilitate cell lysis, protein extraction, subcellular fractionation, organelle isolation and chromatin IP.
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Supporting reagents and kits to stabilize functional enzymes and proteins for storage, solubilize inclusion bodies for recombinant protein purification, and optimize buffer conditions for refolding denatured protein structures.
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High-purity, low-peroxide, surfactant solutions and detergent solids for use in cell lysis reagent formulation, protein solubilization procedures, wash buffers for ELISA, and other protein research methods.
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Affinity columns and precipitation reagents to remove detergents and surfactant micelles from protein extracts and other processed biological samples; eliminate detergent interference to improved compatibility with downstream applications.
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