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Enrich phosphoproteins for analysis in Western blotting or mass spectrometry.
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- Specific - low nonspecific protein contamination from complex biological samples, such as cell culture lysate and mouse tissue extract
- Fast - easy spin format enables enrichment of phosphorylated proteins in less than 2 hours
- Superior Yield - acheives higher yields than other commercially available kits (Table 1)
- Convenient format - complete kit offering pre-dispensed spin columns, buffers, reagents and ultrafiltration columns for enrichment
- Compatible - downstream applications include mass spectrometry, Western blotting, and 2D-PAGE
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Phosphorylation is one of the most frequently occurring post-translational modifications in proteins and it regulates nearly all intracellular biological events including, signal transduction, protein-protein interaction, protein stability, protein localization, apoptosis, and cell cycle control. Detecting changes in protein phosphorylation can be a difficult task, because of the transient labile state of the phosphate group. Furthermore, low phosphoprotein abundance and poorly developed phospho-specific antibodies also contribute to difficulties in phosphoprotein detection. Recent advances in Mass Spectrometry technology in combination with phosphoprotein enrichment using immobilized metal affinity chromatography (IMAC) have resulted in greater resolution of the phosphoproteome.
The Thermo Scientific Pierce Phosphoprotein Enrichment Kit is designed for the efficient enrichment of phosphorylated proteins derived from mammalian cells and tissues. The proprietary resin and buffer composition produces superior yields with negligible non-specific binding. The kit is offered in a convenient spin format and is compatible with downstream applications including Western blotting, Mass Spec, 2D-PAGE, and protein arrays.
| Table 1. The Pierce Phosphoprotein Enrichment Kit provides higher phosphoprotein yields in less time than other kits. |
| Kit |
Phosphoprotein
Yield (µg)†
|
Enrichment Time
(Hours)
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| Our Phosphoprotein Enrichment Kit |
300
|
1.5
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| Competitor Q Kit |
88
|
4.5
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| Competitor I Kit |
52††
|
3.5
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| Competitor C Kit |
160
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3
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| Competitor E Kit |
Too dilute to determine
|
5
|
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† From 2 mg total protein; †† Based on maximum 1 mg load per manufacturer’s protocol
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Figure 1: Pierce Phosphoprotein Enrichment Kit enriches phosphoproteins from complex biological samples with low non-specific binding. |
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Figure 2: Pierce Phosphoprotein Enrichment Kit provides high specificity. |
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Figure 3: Pierce Phosphoprotein Enrichment Kit efficiently enriches phosphoproteins from mouse liver extract. |
Related Resources and Products
Phosphoprotein Enrichment Kit FAQ
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Halt Protease Inhibitor Single-Use Cocktail
Protein Stabilizing Cocktail
Ordering Information
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 Certificate of Analysis  Product Instructions  MSDS |
| Buy |
Product # |
Description |
Pkg. Size |
Files |
Price |
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Add
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90003 |
Pierce Phosphoprotein Enrichment Kit
| Includes: | | | Phosphoprotein Enrichment Columns (1 ml resin bed) | 10 ea. | | Lysis/Binding/Wash Buffer | 325 ml | | Elution Buffer | 60 ml | | CHAPS | 1 g | | Protein Concentrators (7 ml/9K MWCO) | 10 ea. | | White Column Caps | 10 ea. | |
Kit |
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$430.00 |
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