|
|
|
Fast Western Blot Kits, SuperSignal West Pico  |
 |
|
|
|
The increased Western blot sensitivity you need with speed you want.
The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Pico Substrate contains optimized reagents and protocol that allows picogram levels of detection and shortens typical Western blot development to less than 1 hour after the gel transfer.
These Fast Western Blot Kits require minimal hands-on time and yield results comparable to classic Western blotting with SuperSignal West Pico Chemiluminescent Substrate. The kits contain all the reagents necessary to complete a Western blot being probed with either a mouse or rabbit primary antibody. Like other Western blotting methods, the included SuperSignal West Pico Substrate produces a chemiluminescent signal that is detectable using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed.
Highlights of the Pierce Fast Western Blot Kit, SuperSignal West Pico Substrate:
- Fast – picogram levels of detection in about 1 hour
- Convenient – no expensive hardware or vacuum required; no clogging issues
- Simple – optimized protocol makes Western blot analysis easier than ever
- Economical – cost as little as $14 per blot; no expensive consumables or extra equipment
- Excellent stability – kit is stable for 1 year stored at 4°C
- Easy – complete kits contain all components needed to block, probe and develop a blot with your mouse or rabbit primary antibody
About the Fast Western Blot Kits
The Pierce Fast Western Blot Kits are reagent-based system that provide optimized reagents for blocking, antibody dilution and detection of Western blots. The Fast Western Blot Kit protocol is quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other popular rapid Western blotting systems.
| Comparision of other Western blotting protocols to the Thermo Scientific Pierce Fast Western System. This table provides a comparison of the steps and times required to develop Western blot, after the transfer, using the Pierce Fast Western reagent-based system and instrument-based system from Millipore Corporation. |
Protocol Steps |
Classical protocol |
Pierce Fast Western Blot Kit |
SNAP i.d.* |
| Blocking/Pretreatment |
60 min |
- - |
- - |
| Incubation with Primary |
60 min |
30 min |
10 min |
| Wash |
45 min |
- - |
1 min |
| Incubation with Secondary |
60 min |
10 min |
10 min |
| Wash |
45 min |
15 min |
1 min |
| Total Time |
270 min |
55 min |
22 min |
| Key limitations |
• Preparation of fresh non-fat dry milk blocking buffer each time |
• For use with mouse or rabbit primary antibodies |
• Only 2 full-sized blots per cycle. Strips limited by the format of the blot holders available
• Reliable vacuum source required |
| The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Pico Substrate is a cost-effective means to obtain Western blot results quickly. This table compares the cost of reagents and required consumables used in a classical Western blot protocol, the Pierce Fast Western Blot Kit reagent-based system and Millipore SNAP i.d. system. |
Western blot reagents |
Classical protocol |
Pierce Fast Western System |
SNAP i.d. |
| blot holder for instrument |
- - |
- - |
$7.33 |
| blotting membrane (NC) |
$2.48 |
$2.48 |
$2.48 |
| blocking buffer |
$0.02 |
- - |
$1.32 |
| wash buffer (TBST) |
$1.48 |
- - |
$1.48 |
| secondary antibody conjugate |
$0.15 |
- - |
$0.15 |
| detection substrate (SuperSignal West Pico) |
$4.30 |
- - |
$4.30 |
| film (5x7) |
$1.01 |
$1.01 |
$1.01 |
| kit's price per blot |
- - |
$7.92 |
- - |
| Total price per blot |
$9.44 |
$13.44 |
$18.07 |
*Prices based on 2009 Thermo Scientific Pierce Western Blot Reagents and using SuperSignal West Pico Chemiluminescent Substrate as the detection reagent for comparable levels of sensitivity. |
Related Products:
Membranes and Filter Paper
CL-Xposure Film for Western blotting
Pierce Western Blot Signal Enhancer – It's like intensifying screens in a bottle
Restore PLUS Western Blot Stripping Buffer
Browse all Pierce Western Blotting Kits
|
Ordering Information
|
 Certificate of Analysis  Product Instructions  MSDS |
| Buy |
Product # |
Description |
Pkg. Size |
Files |
Price |
|
Add
|
35065 |
Fast Western Kit, SuperSignal West Pico, Mouse
Sufficient reagents to perform 5 Western blots (8cm × 10cm) probed with mouse primary antibodies. |
kit |
|
$98.00 |
|
Add
|
35060 |
Fast Western Kit, SuperSignal West Pico, Mouse
Sufficient reagents to perform 20 Western blots (8cm × 10cm) probed with mouse primary antibodies.
Includes:
Fast Western Antibody Diluent, 400ml
Fast Western 10X Wash Buffer, 200ml
Fast Western Mouse Optimized HRP Reagent, Pico, 20ml
SuperSignal West Pico Luminol/Enhancer Solution Reagent, 100ml
SuperSignal West Stable Peroxide Solution, 100ml
|
kit |
|
$198.00 |
|
Add
|
35066 |
Fast Western Kit, SuperSignal West Pico, Rabbit
Sufficient reagents to perform 5 Western blots (8cm × 10cm) probed with rabbit primary antibodies. |
kit |
|
$98.00 |
|
Add
|
35061 |
Fast Western Kit, SuperSignal West Pico, Rabbit
Sufficient reagents to perform 20 Western blots (8cm × 10cm) probed with rabbit primary antibodies.
Includes:
Fast Western Antibody Diluent, 400ml
Fast Western 10X Wash Buffer, 200ml
Fast Western Rabbit Optimized HRP Reagent, Pico, 20ml
SuperSignal West Pico Luminol/Enhancer Solution Reagent, 100ml
SuperSignal West Stable Peroxide Solution, 100ml
|
kit |
|
$198.00 |
|
|
|
|
|
|
|
|
|
