Obtain your Western blot results in about an hour without buying an instrument.

The Thermo Scientific Pierce Fast Western Blot Kit, ECL Substrate contains optimized reagents that shorten the time to perform a typical Western blot from 4 hours down to approximately 55 minutes. The kit provides all the reagents necessary to complete a Western blot being probed with a mouse or rabbit primary antibody. The protocol requires minimal hands-on time and yields results comparable to classic Western blotting with ECL. The included Pierce ECL Substrate produces a chemiluminescent signal, which is detected using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the primary antibody and immunodetection reagents and reprobed.

About the Fast Western Blot Kits, ECL Substrate

The Pierce Fast Western Blot Kit is reagent-based system that provides optimized reagents for blocking, antibody dilution and detection of Western blots with Pierce ECL substrate. The protocol is a quick, efficient and economic way to obtain Western blot results without the hassle of buying an instrument and the consumables required by other popular rapid Western blotting systems. No vacuum pump is needed so there are never any clogged lines or membranes that can occur when using instrument-based systems. Because there is no apparatus, there is no need to buy disposable blotting trays that limit the number of blots processed at one time.

Highlights of the Pierce Fast Western Blot Kit, ECL Substrate:

• Fast – all the sensitivity of Pierce ECL substrate and saving 4-5 hours per blot
• Convenient – no expensive hardware or vacuum required; no clogging issues
• Simple – optimized protocol makes Western blot analysis easier than ever
• Economical – cost as little as $8 per blot; no expensive consumables or extra equipment
• Excellent stability – kit is stable for 1 year stored at 4 °C
• Easy – complete kit gives you all the components you need to block and probe and develop a blot with your mouse or rabbit primary antibody

Comparable results obtained with classic Western blotting protocol and Thermo Scientific Pierce Fast Western Blot Kit, ECL Substrate. The detection sensitivity for various target proteins was compared using the Fast Western Blot Kit and the classical Western blot protocol, Panels A and B contained 10, 2, 0.4 and 0.08 µg of protein from A549 cell lysates in lanes 1-4, respectively. Panels C and D contained 2, 1, 0.5, 0.25 and 0.125 µg of 293T cell lysate in lanes 1-5 of Panel C and 25, 12.5, 6.25, 3.1 and 0.39 µg in lanes 1-5 of Panel D. Protein detection was achieved as follows: Panel A. Mouse anti-Cyclin D1 antibody (BD Pharmingen) on PVDF. Panel B. Rabbit anti-Beta Catenin antibody (LabVision) on PVDF. Panel C. Mouse anti-GAPDH antibody (Millipore) on nitrocellulose. Panel D. Rabbit anti-Cdk2 antibody (Upstate) on PVDF.

Researcher generated data using the Fast Western Blot Kit, ECL Substrate
	Multiplex Experiment with Thermo Scientific Pierce Fast Western Blot Kit, ECL Substrate. C57BL/6 mice bone marrow cells were stimulated with M-CSF (50 ng/ml) and RANKL (50 ng/ml) to induce differentiation into osteoclasts. Total cell lysates on PVDF were probed with 0.2 µg/m rabbit anti-hsp90 and mouse anti-cathepsin K (Santa Cruz Biotechnology). Data generated by Lai-Chu Wu, Department of Molecular and Cellular Biochemistry, Ohio State University, Columbus, Ohio.
	Fast detection of His-tagged SAM68 protein purified using a Ni column from E. coli lysate. Lane 1. Lysate only, Lane 2. Column flow-through, Lanes 3-4. Wash and Lanes 5-8. Elution fractions. Fractions on PVDF were probed with rabbit anti-His-tag primary antibody (Santa Cruz Biotechnology). Data generated by Brenda Suh-Lailam and Joan Hevel, Department of Chemistry and Biochemistry, Utah State University, Logan, Utah.

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