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YOU ARE HERE:  Browse Product Catalog > Target Specific Assay Kits > ELISPOT Kits > Human IFN gamma ELISPOT Kit  


Human IFN gamma ELISPOT Kit 

Endogen’s Human IFNγ ELISPOT (enzyme-lined immunospot) Kit analyzes interferon gamma (IFNγ) production at the single cell level. A spot forms at the site where secreted cytokine is bound, is counted (either using a dissection microscope or automated ELISPOT reader) and then compared to the number of cells added to the microplate. This allows the frequency of cells secreting the cytokine to be calculated. One spot is equal to one molecule-secreting cell.

Features and Benefits:
  • Consistent Membrane Coating - stringent quality control procedures ensure well-to-well and within-well consistency
  • Optimal Plate Blocking - an optimized blocking procedure increases signal-to-noise ratios and lowers background values
  • Well-Characterized Antibody Pairs - specific, high-affinity antibody pairs provide optimal sensitivity
  • Ease-of-Use - pre-coated plates, pre-titered reagents, and a simple assay procedure make testing simple
  • Short Assay Time - development of spots is completed in just 2 ½ hours following cell culture, offering fast, accurate results

List of Reagents

  • Precoated 96-well membrane plates, 2 each
  • Biotin-labeled Detection Antibody
  • Streptavidin-AP Conjugate
  • Streptavidin-AP Diluent
  • 10X Wash Buffer
  • NBT/BCIP Substrate Solution
How An ELISPOT Works
  • Species: Human
  • Analyte: Human IFN gamma 
  • Format: 96-well PVDF membrane bottom plate coated with IFN gamma antibody 
  • Total Assay Time:3 hours
  • Sample Size: 50 µl/well

Instrumentation Options:
For more information on our instruments for reading ELISAs, visit www.thermo.com/readingroom

Ordering Information
 Certificate of AnalysisCertificate of Analysis   Product InstructionsProduct Instructions   MSDSMSDS
Buy Product # Description Pkg. Size Files Price
Add ELHIFNG Human IFN gamma ELISPOT Kit 2 plate MSDS for product # ELHIFNG Human IFN gamma ELISPOT Kit Certificate of Analysis $501.00

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