For efficient high-throughput removal of salt and small molecules.
The effective removal of salt and small molecules from samples is a critical step in identifying and quantifying large number of proteins in biological systems. Furthermore, sample prep automation is a desirable goal for enabling high throughput proteomics analysis. The Zeba 96-well Spin Desalting Plates provide high throughput removal of salt and small molecules from samples thus preparing them for array of downstream analyses such as mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development.
Highlights of Zeba 96-well Spin Desalting Plates:
- Desalt 96 samples in 5 minutes
- No screening fractions for sample or waiting for samples to emerge by gravity flow
- Exceptional protein recovery
- Easy-to-use with no cumbersome plate preparation or equilibration
- Minimal sample dilution
The Zeba 96-well Spin Desalting Plates contain a high-performance resin offering exceptional desalting and protein recovery characteristics. Small sample volumes (20–100µl) can be processed providing exceptional protein recovery and > 95% removal of salts and other small molecules (<1,000 Da, e.g., DTT, Biotin, FITC, Biotin-FITC etc.). The Zeba 96-well Spin Desalting Plates require no resin dispensing or hydration. One plate of 96 samples can be processed in 5 minutes.
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| The Zeba 96-well Spin Desalting Plates are easy to use. |
| Protein recovery and Desalting Efficiency: Protein samples (1mg/ml) were prepared in 1.0M NaCl and 100µl samples were desalted using Zeba Spin Desalting Plates. Results were analyzed by BCA Protein Assay and conductivity measurements. |
| Protein |
Protein Recovery % |
NaCl Removed |
| BSA (66 kDa) |
98.5 |
>95 |
| a-Lactalbumin (14.1 kDa) |
91.5 |
>95 |
| Ubiquitin (8.6 kDa) |
85 |
>95 |
Related Products:
Desalting Product Selection Guide
Zeba Desalting Spin Columns (sample volumes 2µl–4ml)
Zeba Desalting Chromatography Cartridges (sample volumes 50µl–1.5ml)
Pierce Centrifuge Columns (empty)
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