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YOU ARE HERE:  Browse Product Catalog > Target Specific Assay Kits > In-Cell ELISA Kits for Specific Targets > GSK3 alpha/beta In-Cell ELISA Kit  


GSK3 alpha/beta In-Cell ELISA Kits 

In-cell ELISAs for measuring relative total GSK3α/β and phospho-GSK3α/β levels.

The Thermo Scientific Pierce GSK3α/β In-Cell ELISA Kits enable the relative quantitation of total and phosphorylated glycogen synthase kinase 3 (GSK3) in whole cells using a 96- or 384-well microplate format. The complete kits include a mouse monoclonal anti-GSK3α/β antibody, rabbit monoclonal anti-phospho-(Ser21/Ser9) GSK3α/β antibody, detection reagents and all buffers required for either colorimetric or near infrared detection.

 

Highlights of the In-Cell ELISA Kits:

  • Two reliable detection formats available: (Learn more)
    • colorimetric detection kit for use with standard microplate readers
    • near infrared fluorescent detection kit for use with IR imaging system
  • Whole-cell detection requires no cell lysis, protein extraction or special sample preparation
  • Provides better data and higher throughput than quantitative Western blotting
  • Less costly (one well vs. one lane) than Western blotting

 

Related Products and Links:
ELISA reagent reservoirs and sealing tape
Browse all In-Cell ELISA Kits for specific targets
Basic In-Cell ELISA Detection Kits – basic kit to be used with your primary antibodies

 


Ordering Information
Certificate of AnalysisCertificate of Analysis   Product InstructionsProduct Instructions   MSDSMSDS
Buy Product # Description Pkg. Size Files Price
Add 62217 GSK3 a/ß Colorimetric In-Cell ELISA Kit
Sufficient materials for 1 x 96 wells
Includes:
Blocking Buffer, 50 ml
20X Tris Buffered Saline, 50 ml
Surfact-Amps 20 (10% Tween-20 Detergent), 10 ml
Surfact-Amps X-100 (10% Triton X-100 Detergent), 10 ml
HRP Conjugate, 0.14 ml
TMB Substrate, 58 ml
TMB Stop Solution, 55 ml
Janus Green Whole-Cell Stain, 50 ml
Elution Buffer, 2 x 30 ml
Thin Plate Seal Assembly, 8 each
Anti-Phospho GSK3 α/β (S21/9) Antibody
Anti-GSK3 Antibody
kit Product Instructions MSDS for product # 62217 GSK3 a/ß Colorimetric In-Cell ELISA Kit $339.00
Add 62222 GSK3 a/ß Near Infrared In-Cell ELISA Kit
Sufficient materials for 1 x 96 wells
Includes:
Blocking Buffer, 100 ml
20X Tris Buffered Saline, 50 ml
Surfact-Amps 20 (10% Tween-20 Detergent), 10 ml
Surfact-Amps X-100 (10% Triton X-100 Detergent), 10 ml
DyLight 680 Goat Anti-Mouse IgG (H+L), 55 µl
DyLight 800 Goat Anti-Rabbit IgG (H+L), 55 µl
Thin Plate Seal Assembly, 8 each
Anti-Phospho GSK3 α/β (S 21/9) Antibody
Anti-GSK3 Antibody
kit Product Instructions MSDS for product # 62222 GSK3 a/ß Near Infrared In-Cell ELISA Kit $339.00

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