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AKT In-Cell ELISA Kits |
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In-cell ELISAs for measuring relative total AKT and phospho-AKT levels.
The Thermo Scientific Pierce AKT In-Cell ELISA Kits enable the relative quantitation of total and phosphorylated Akt family of serine/threonine-specific protein kinases (AKT1,2 and 3) in whole cells using a 96- or 384-well microplate format. The complete kits include a mouse monoclonal anti-AKT antibody, rabbit monoclonal anti-phospho-(Ser473) AKT antibody, detection reagents and all buffers required for either colorimetric or near infrared detection.
Highlights of the In-Cell ELISA Kits:
- Two reliable detection formats available: (Learn more)
- colorimetric detection kit for use with standard microplate readers
- near infrared fluorescent detection kit for use with IR imaging system
- Whole-cell detection requires no cell lysis, protein extraction or special sample preparation
- Provides better data and higher throughput than quantitative Western blotting
- Less costly (one well vs. one lane) than Western blotting
Related Products and Links:
ELISA reagent reservoirs and sealing tape
Browse all In-Cell ELISA Kits for specific targets
Basic In-Cell ELISA Detection Kits – basic kit to be used with your primary antibodies
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Ordering Information
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 Bulk quantities: Inquire  Certificate
of Analysis  Product
Instructions  MSDS |
| Product # |
Description |
Pkg. Size |
Files |
Price |
| 62215 |
AKT Colorimetric In-Cell ELISA Kit
Sufficient materials for 1 x 96 wells
Includes:
Blocking Buffer, 50 ml
20X Tris Buffered Saline, 50 ml
Surfact-Amps 20 (10% Tween-20 Detergent), 10 ml
Surfact-Amps X-100 (10% Triton X-100 Detergent), 10 ml
HRP Conjugate, 0.14 ml
TMB Substrate, 58 ml
TMB Stop Solution, 55 ml
Janus Green Whole-Cell Stain, 50 ml
Elution Buffer, 2 x 30 ml
Thin Plate Seal Assembly, 8 each
Anti-Phospho AKT (S473) Antibody
Anti-AKT Antibody |
kit |
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$339.00
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| 62220 |
AKT Near Infrared In-Cell ELISA Kit
Sufficient materials for 1 x 96 wells
Includes:
Blocking Buffer, 100 ml
20X Tris Buffered Saline, 50 ml
Surfact-Amps 20 (10% Tween-20 Detergent), 10 ml
Surfact-Amps X-100 (10% Triton X-100 Detergent), 10 ml
DyLight 680 Goat Anti-Mouse IgG (H+L), 55 µl
DyLight 800 Goat Anti-Rabbit IgG (H+L), 55 µl
Thin Plate Seal Assembly, 8 each
Anti-Phospho AKT (S473) Antibody
Anti-AKT Antibody |
kit |
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$339.00
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