Easily prepare your own high-performance fluorescent probes.

The DyLight Antibody Labeling Kits provide for fast and efficient fluorescent labeling of antibodies for use in fluorescence methods. Choose a kit for labeling with any one of nine popular DyLight Fluorescent Dyes, which have absorption spectra ranging from 400 nm to 770 nm and match the principal output wavelengths of common fluorescence instrumentation. DyLight Conjugates exhibit higher fluorescence intensity and photostability than Alexa Fluor*, Cy* and LI-COR* Dyes in many applications and remain highly fluorescent over a broad pH range.

Standard and microscale kit sizes are offered for each DyLight Fluor. The standard size Antibody Labeling Kits contain all necessary components to perform three separate labeling reactions using 1 mg of IgG or similar quantities of other proteins. The Microscale Antibody Labeling Kits contain all the necessary components to perform five separate labeling reactions using 100 µg of IgG. Both kits sizes include the amine-reactive, DyLight NHS-ester Reagent in convenient single-use vials, as well as purification resin and spin columns for preparation of ready-to-use conjugate.

Labeling Kit Highlights:

• Fast – label and purify antibody with any DyLight Fluor in about one hour
• Amine-specific labeling – the NHS-ester DyLight Dyes efficiently label antibodies and other purified proteins at primary amines (-NH2)
• Optimized procedure – following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence
• Single-use DyLight Reagent – no need to weigh tiny amounts of reagent powder; kits provide separate, single-use vials of reagent
• Efficient purification – kits include purification resin and easy-to-use spin columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery

DyLight Fluorophore Highlights:

• Bright fluorescence – intense emission provides superior sensitivity and requires less conjugate
• Narrow emission spectra – negligible bleed-through between fluorophore channels enables multi-color detection
• Excellent photostability – exceptional resistance to photobleaching enables fluorescence imaging under the most demanding conditions (e.g., structured illumination, 4Pi microscopy)
• Buffer stability – conjugated fluorophores are completely stable at pH 4-9
• Instrument compatibility – excitation and emission spectra correspond with filter sets and laser settings of all popular fluorescence instrumentation

Emission spectra of Thermo Scientific DyLight Fluors 350-800. Line colors represent the approximate visible colors of the wavelength maxima. Learn more... DyLight Dye spectral properties (opens new window)

Kit Details:

Procedure summary for Thermo Scientific DyLight Antibody Labeling Kits. The protocol is simple and provides efficient labeling, exceptional clean-up and excellent antibody recovery.

DyLight Antibody Labeling Kits provide outstanding recovery. The percent recovery for DyLight Antibody Labeling Kits is the average for 16 labeling reactions using three different antibodies. The percent recovery for DyLight Microscale Antibody Labeling Kits is the average for 13 labeling reactions using three different antibodies.

Related Products and Resources:

• Complete DyLight Fluor Product Guide
• All Fluorescent Labeling Reagents and Kits
• DyLight Fluor Secondary Antibody Conjugates
• DyLight Fluor Streptavidin and NeutrAvidin Conjugates
• DyLight Fluorescent Western Blotting Kits and Fluorescent MW Markers
• Krypton Fluorescent Protein Stains

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