Halt Phosphatase Inhibitor Cocktail safeguards against serine, threonine and protein tyrosine phosphatase activities

Phosphorylation and dephosphorylation is a molecular on/off switch that regulates a number of key biological pathways within the cell, including signal transduction, cell division, and apoptosis. The Halt Phosphatase Inhibitor Cocktail preserves phosphorylation of proteins in cell lysates and tissue extracts. The cocktail contains a mixture of four phosphatase inhibitors of broad specificity, including sodium fluoride, sodium orthovanadate, sodium pyrophosphate and -glycerophosphate. Unlike other commercially available phosphatase inhibitor cocktails which protect against either serine/threonine phosphatases or protein tyrosine phosphatases, the Halt Phosphatase Inhibitor Cocktail protects phosphoproteins from both families of phosphatases (Figure 1). Conveniently packaged in a 100X format, the cocktail is stable at 4°C and is compatible with common protein assays, including the BCA Protein Assay Kit (#23225) and Bradford-based assays such as Coomassie Plus-The Better Bradford Assay (#23236).

• All in one format – The Halt Phosphatase Inhibitor Cocktail inhibits both serine/threonine as well as protein tyrosine phosphatases
• Compatible with standard protein assays – The BCA Protein Assay Kit and Coomassie Plus-The Better Bradford Assay
• User friendly storage at 2-8°C – The Halt Phosphatase Inhibitor Cocktail does not require frozen storage
• Convenient size – 1 ml of cocktail protects up to 100 ml of sample

		Figure 1: Effect of Halt Phosphatase Inhibitor Cocktail on phosphorylation of MEK, MAP Kinase 42/44, and Stat3 in HeLa cell lysate. Hela cells were lysed in the absence (-) and presence (+) of a 1X concentration of the Halt Phosphatase Inhibitor cocktail. Cell lysates were analyzed by Western blot for total and phosphorylated protein as indicated. (A) MEK and phosphorylated MEK (PMEK), (B) MAP Kinase and PMAP Kinase, (C) STAT3 and PSTAT3. The proteins are phosphorylated on serine, threonine/tyrosine, and tyrosine, respectively. A marked increase in phosphorylation of each of the three proteins was observed.

• The Halt Phosphatase Inhibitor Cocktail does not contain protease inhibitors. If necessary, use a broad-spectrum protease inhibitor, such as the Halt Protease Inhibitor Cocktail, in addition to the Phosphatase Inhibitor Cocktail.
• The Halt Phosphatase Inhibitor Cocktail interferes with immobilized metal chelate affinity chromatography and 2-D gel electrophoresis. Either dialyze or desalt sample to effectively remove inhibitors from cell extracts before performing such procedures.
• The Halt Phosphatase Inhibitor Cocktail is supplied as a 100X concentrate that is effective when used at a final concentration of 1X; however, samples that contain particularly high levels of phosphatase activity may require a final concentration of 2-3X.
• The Halt Phosphatase Inhibitor Cocktail is compatible with all Pierce Cell Lysis Solutions.
• The Halt Phosphatase Inhibitor Cocktail is does not affect alkaline phosphatase activity.

Ordering Information

Certificate of Analysis   Product Instructions   MSDS

Buy Product # Description Pkg. Size Files Price Add 78420 Halt Phosphatase Inhibitor Cocktail A mixture of four phosphatase inhibitors Including: sodium fluoride, sodium orthovanadate, sodium pyrophosphate and beta-glycerophosphate. 1 ml $164.00 Add 78426 Halt Phosphatase Inhibitor Cocktail 5 x 1 ml $490.00 Add 78427 Halt Phosphatase Inhibitor Cocktail 10 ml $671.00 Add 78428 Halt Phosphatase Inhibitor Single-Use Cocktail Each 100 µl microtube contains sufficient cocktail to treat 10 ml of lysate 24 x 100 µl $248.00