Charge through your purifications with ease using membrane-based and resin-free ion exchange spin columns

Highlights: Fast and simple – membrane-based spin format eliminates column packing Convenient and expandable – centrifugal format enables convenient processing of multiple samples in parallel Robust – membrane adsorber spin columns do not crack or run dry Low bed volume – small membrane adsorber bed volumes make working with low buffer volumes possible, leading to concentrated elution fractions

Ion exchange chromatography separates molecules based on differences in their accessible surface charges. This technique is widely used in the pre-fractionation or purification of a target protein from crude biological samples. Novel membrane-based ion exchange chromatography is attracting attention because of its advantages over resin-based column chromatography. Membrane-based ion exchange chromatography has shorter diffusion times than resin-based chromatography. Interactions between molecules and active sites on the membrane occur in a convective manner through pores, which shortens the diffusion time compared with fluid inside the pores of a resin particle. Also, the relatively mild binding and eluting conditions of this separation method produce high protein recovery with intact biological activity.

Thermo Scientific Pierce* Strong Cation and Anion Ion Exchange Spin Columns use the membrane-adsorber technology as a chromatographic matrix to fractionate proteins based on their charge differences. The membrane adsorbers have a highly porous structure with pores larger than 3,000 nm, providing proteins easy access to the membrane’s charged ligands. Therefore, adsorptive membranes maintain high efficiencies at high-flow rates and when fractionating large biomolecules with small diffusivities.

Protocol Summary

• Mini, offering binding capacities of 4 mg (500 µl)
• Maxi, offering binding capacities of 80 mg (20 ml)

• Pre-fractionation of proteins in lysate
• Scouting purification conditions for new protein preparation protocols
• Removal of endotoxins from monoclonal antibodies
• Polishing His-tagged proteins after metal chelate chromatography
• Purification and concentration of proteins and viral particles
• Purification of antibodies from serum, ascites or tissue culture supernatant
• Removal of detergent from protein solutions
• Sample preparation before 1D or 2D PAGE
• Purification of phosphopeptides before MS analysis

Ordering Information	Certificate of Analysis   Product Instructions   MSDS
Buy Product # Description Pkg. Size Files Price Add 90008 Pierce Strong Cation Exchange Spin Column, Mini 24 spin columns $115.00 Add 90009 Pierce Strong Cation Exchange Spin Column, Maxi 8 spin columns $219.00 Add 90010 Pierce Strong Anion Exchange Spin Column, Mini 24 spin columns $115.00 Add 90011 Pierce Strong Anion Exchange Spin Column, Maxi 8 spin columns $219.00