An excellent alternative to radioisotopic detection in Northern and Southern blots, ribonuclease protection and electrophoretic mobility shift assays.

The Thermo Scientific Chemiluminescent Nucleic Acid Detection Module is a complete system for detection of biotin-labeled nucleic acid probes in various membrane blotting applications. Nearly any DNA or RNA method based on nitrocellulose or nylon membranes and biotinylated probes can be detected with sensitivity equivalent to traditional radioactive (32P) systems.

The module consists of the essential detection components used in the Thermo Scientific North2South Kit and Thermo Scientific LightShift EMSA Kit. The system includes an enhanced luminol substrate for horseradish peroxidase (HRP) with optimized blocking and wash buffers that provide for fast, trouble-free detection on film or by CCD camera. Obtain publication-quality blots having excellent signal and low background.

Highlights:

• Fast and sensitive – achieve the same speed and detection levels you’ve come to expect with Thermo Scientific North2South and LightShift Kits
• Versatile – kit protocol (included) is applicable to any biotin-based nucleic acid detection system: Northerns, Southerns, EMSA, RPAs and colony lifts
• Flexible – kit contains only the critical detection components for those who already have biotin-labeled probes and an optimized hybridization method developed

References:

1. Matata, B. M. and Galiñanes, M. (2002). J. Biol Chem. 277(3), 2330–2335.
2. Oltra, E., et al. (2003). Endocrinology 144(7), 3148-3158.

Related Products and Resources:

Biodyne B Nylon Membrane
LightShift Chemiluminescent EMSA Kit
North2South Chemiluminescent Detection of Nucleic Acids
North2South Biotin Random Prime DNA Labeling Kit
Biotin 3´ End DNA Labeling Kit