Premier disposable ultrafiltration centrifugal devices for concentration, diafiltration and buffer-exchange of biological samples.
Thermo Scientific Pierce Protein Concentrators are disposable centrifugal devices for high-performance concentration, diafiltration or buffer-exchange of biological samples.
Pierce Protein Concentrators are available with 9K, 20K and 150K MWCOs in 7mL and 20mL capacities and are compatible with swinging-bucket and fixed-angle rotors. These centrifugal concentrators provide efficient concentration of biological samples containing proteins (antigens, antibodies and enzymes), nucleic acids (DNA) and micro organisms (viral particles).
Highlights of Pierce Protein Concentrators:
Superior protein concentration and recovery – achieve > 100-fold protein concentration in less than 45 minutes with > 90% protein recovery
Convenient – spin formats for concentrating 1 to 20mL of sample
Instrument-compatible – use swinging-bucket or fixed-angle rotors; collect sample without invert spinning
Versatile – Pierce Protein Concentrators are the easy way to perform:
Protein concentration with tissue culture media, antiserum or monoclonal antibody preps
Concentration of protein peaks following gel-permeation chromatography
Removal of unincorporated protein label
Concentration and desalt/buffer-exchange after eluting protein from ion-exchange, hydrophobic interaction (HIC), metal chelate or affinity chromatography columns
About Pierce Protein Concentrators:
Pierce Protein Concentrators have a unique conical high-performance design that overcomes many problems associated with traditional protein concentration, providing a high degree of concentration in a single spin while minimizing polarization and adsorption at the membrane surface. Their regenerated cellulose membranes are accurately rated for molecular-weight cutoffs (MWCO) and routinely provide > 90% recovery of proteins larger than the membrane MWCO. Concentration factors of > 100-fold can be obtained in less than 45 minutes. Thermo Scientific Pierce Portein Concentrators also allow accurate control of the dead-stop volume and final concentration factor, providing reliable and consistent sample processing, especially with low-concentration samples that are often lost or damaged with other centrifugal concentrator devices.
Thermo Scientific Pierce Protein Concentrators make sample loading and recovery easy. Simply load up to 20mL of sample into the large concentration device (or up to 7mL with the smaller unit), cap the device and insert it into a fixed angle or swinging bucket rotor. Centrifuge the sample for the desired amount of time and rpm and then recover the concentrated sample. No inverted spin or membrane washing is required due to the unique conical design.
Molecular Weight (MW) vs Protein Recovery
Concentrator
BSA
(66,000 kDa)
Lysozyme (14,000 kDa)
Ubiquitin
(8,700 kDa)
Thermo Scientific Pierce Protein Concentrator, 9K MWCO
91%
98%
96%
Millipore Amicon* Ultra Unit, 10K MWCO
90%
93%
91%
Thermo Scientific Pierce Protein Concentrators provide exceptional recovery of low-concentration samples. Samples of bovine serum albumin (BSA), lysozyme and ubiquitin at approximately 0.02mg/mL starting concentration were centrifuged in Pierce Protein Concentrators and other supplier’s concentrators at 3,000 x g until a 15- to 25-fold decrease in sample volume was achieved. Samples were recovered without membrane washing and protein recovery was determined by the Thermo Scientific Pierce BCA Protein Assay. Results are unaffected by centrifugation rate. Results were similar with both 7mL and 20mL Protein Concentrators. Similar or higher recovery values were obtained with the Pierce Protein Concentrators at higher protein loads (data not shown).
Sample recovery and concentration compared to starting concentration using Thermo Scientific Pierce Protein Concentrators.
Thermo Scientific Pierce Protein Concentrators are disposable centrifugal devices for high-performance concentration, diafiltration or buffer-exchange of biological samples. 
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