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YOU ARE HERE:  Browse Product Catalog > Dialysis and Desalting > Pierce Concentrators  


Pierce Protein Concentrators 

Premier disposable ultrafiltration centrifugal devices for concentration, diafiltration and buffer-exchange of biological samples.

Pierce Protein Concentrators have superior recovery compared to leading protein centrifugation conentration devicesThermo Scientific Pierce Protein Concentrators effectively combine simplicity, speed, capacity and recovery to yield enhanced concentration, purification and separation of proteins – even with dilute protein samples. Pierce Concentrators are available with 9K, 20K and 150K MWCOs in 7 ml and 20 ml capacities and are compatible with swinging-bucket and fixed-angle rotors. These centrifugal concentrators provide efficient concentration of biological samples containing proteins (antigens, antibodies and enzymes), nucleic acids (DNA) and micro organisms (viral particles).

About Pierce Protein Concentrators:

Pierce Protein Concentrators have a unique conical high-performance design that overcomes many problems associated with traditional protein concentration, providing a high degree of concentration in a single spin while minimizing polarization and adsorption at the membrane surface. Their regenerated cellulose membranes are accurately rated for molecular-weight cutoffs (MWCO) and routinely provide > 90% recovery of proteins larger than the membrane MWCO. Concentration factors of > 100-fold can be obtained in less than 45 minutes. The Pierce Concentrators also allow accurate control of the dead-stop volume and final concentration factor, providing reliable and consistent sample processing, especially with low-concentration samples that are often lost or damaged with other centrifugal concentrator devices.

 

Highlights of Pierce Protein Concentrators:

  • Superior protein concentration and recovery – achieve > 100-fold protein concentration in less than 45 minutes with > 90% protein recovery
  • Convenient – spin formats for concentrating 1 to 20 ml of sample
  • Instrument-compatible – use swinging-bucket or fixed-angle rotors; collect sample without invert spinning
  • Versatile – Pierce Concentrators are the easy way to perform:
    • Protein concentration with tissue culture media, antiserum or monoclonal antibody preps
    • Concentration of protein peaks following gel-permeation chromatography
    • Removal of unincorporated protein label
    • Concentration and desalt/buffer-exchange after eluting protein from ion-exchange, hydrophobic interaction (HIC), metal chelate or affinity chromatography columns
Small figures can be positioned with title and legend placed alongside.
Thermo Scientific Pierce Protein Concentrators make sample loading and recovery easy. Simply load up to 20 ml of sample into the large concentration device (or up to 7 ml with the smaller unit), cap the device and insert it into a fixed angle or swinging bucket rotor. Centrifuge the sample for the desired amount of time and rpm and then recover the concentrated sample. No inverted spin or membrane washing is required due to the unique conical design.

 

Molecular Weight (MW) vs Protein Recovery
Concentrator BSA
(66,000 kDa)
Lysozyme
(14,000 kDa)
Ubiquitin
(8,700 kDa)
Pierce Concentrator, 9K MWCO
91%
98%

96%

Millipore Amicon* Ultra Unit, 10K MWCO
90%
93%
91%
Vivascience Vivaspin* Unit, 10K MWCO
76%
5%

5%

Thermo Scientific Pierce Concentrators provide exceptional recovery of low-concentration samples. Samples of bovine serum albumin (BSA), lysozyme and ubiquitin at approximately 0.02 mg/ml starting concentration were centrifuged in Pierce Concentrators and other supplier’s concentrators at 3,000 x g until a 15- to 25-fold decrease in sample volume was achieved. Samples were recovered without membrane washing and protein recovery was determined by the Thermo Scientific Pierce BCA Protein Assay. Results are unaffected by centrifugation rate. Results were similar with both 7 ml and 20 ml Pierce Concentrators. Similar or higher recovery values were obtained with the Pierce Concentrators at higher protein loads (data not shown).

 

Sample Recovery and Concentration Compared to Starting Concentration
Protein Concentrator Unit Time
(3000 x g)
Starting Concentration Recovery Fold Concentration
Cytochrome C
12 kDa
7ml/9K
35 min

0.2 mg/ml

96%

137

0.01 mg/ml

88%

129

20ml/9K
45 min

0.2 mg/ml

100%

121

0.01 mg/ml

96%

117

BSA
66 kDa
7ml/20K
35 min

0.2 mg/ml

97%
81

0.01 mg/ml

87%

65

20ml/20K
45 min

0.2 mg/ml

98%
137

0.01 mg/ml

95%

118

 

Related Products and Links:
Dialysis Membrane and Devices
Desalting Columns
Contaminant Removal Products
Concentrated Buffers
BupH Dry Blend Buffer Packs


Ordering Information
Certificate of AnalysisCertificate of Analysis   Product InstructionsProduct Instructions   MSDSMSDS
Buy Product # Description Pkg. Size Files Price
Add 87748 Pierce Concentrators, 9K MWCO, 7ml 10 concentrators Product Instructions $61.00
Add 89884A Pierce Concentrators, 9K MWCO, 7ml
Replaces 89884, iCON Concentrator
25 concentrators Product Instructions $139.00
Add 87749 Pierce Concentrators, 9K MWCO, 20ml 10 concentrators Product Instructions $110.00
Add 89885A Pierce Concentrators, 9K MWCO, 20ml
Replaces 89885, iCON Concentrator
25 concentrators Product Instructions $257.00
Add 87750 Pierce Concentrators, 20K MWCO, 7ml 10 concentrators Product Instructions $61.00
Add 89886A Pierce Concentrators, 20K MWCO, 7ml
Replaces 89886, iCON Concentrator
25 concentrators Product Instructions $139.00
Add 87751 Pierce Concentrators, 20K MWCO, 20ml 10 concentrators Product Instructions $110.00
Add 89887A Pierce Concentrators, 20K MWCO, 20ml
Replaces 89887, iCON Concentrator
25 concentrators Product Instructions $257.00
Add 89920 Protein Concentrators, 150K MWCO, 7ml 10 concentrators Product Instructions $61.00
Add 89922 Protein Concentrators, 150K MWCO, 7ml 25 concentrators Product Instructions $139.00
Add 89921 Protein Concentrators, 150K MWCO, 20ml 10 concentrators Product Instructions $110.00
Add 89923 Protein Concentrators, 150K MWCO, 20ml 25 concentrators Product Instructions $257.00

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