Endogen’s ELISPOT Sets contain the basic components and protocol required to develop ten colorimetric ELISPOT assays for quantitation of the number of cells secreting a specific cytokine. Every ELISPOT Set contains pre-titered Coating and Detection Antibodies, and Streptavidin-AP. All components undergo strict quality control testing. The ELISPOT procedure has been optimized to reduce background and maximize spot number and spot consistency within and between wells.

Highlights

• Species:   Human
• Analyte: TNF α
• List of Reagents:
  • Coating Antibody
  • Detection Antibody
  • Streptavidin-AP

Features / Benefits

• Carefully selected Antibody Pair offering optimal specificity and sensitivity.
• Optimized Procedure designed to maximize signal and coating consistency and reduce background

Assay Time: 2 hrs. 15 min. following incubation of cells with stimulant