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- Species: Mouse
- Analyte: IL-1 alpha
- Format: 96-well strip plate
- Assay Range: 15.6 - 1,000 pg/ml
- Sensitivity: < 6 pg/ml
- Total Assay Time: 3 hours
- Sample Size: 50 µl/well
- Sample Types: Serum; EDTA and Heparin; and Sodium Citrate Plasma; Cell Culture Supernate
- Wavelength: A450 nm minus A550 nm *
*Correcting for optical imperfections in the microplates by subtracting A550 nm is recommended, but not an essential procedure.
Features
- Superior sensitivity - accurately measure as little as 6 pg/ml of mouse IL-1 alpha
- Broad assay range - detect both low and elevated levels with fewer sample dilutions and retests
- Small sample size - generate more results and conserve precious samples
References
- Fantuzzi, G., et al. (1996). Effect of endotoxin in IL-1B-deficient mice. J. Immunol. 157, 291-296.
- Ghayur, T., et al. (1997). Caspase-1 processes IFN-g-inducing factor and regulates LPS-induced IFN-g production. Nature. 386, 619-623.
- Gonzalo, J.A., et al. (1996). Enterotoxin septic shock protection and deficient T helper 2 cytokine production in growth hormone transgenic mice. J. Immunol. 157, 3298-3304.
- Kambayashi, T., et al. (1995). Potential involvement of IL-10 in suppressing tumor-associated macrophages. J. Immunol. 154, 3383-3390.
- Kocikova, A., et al. (1998). Murine langerhans cell cultured under serum-free conditions mature into potent stimulators of primary immune response in vitro and in vivo. J. Immunol. 161, 4033-4041.
- Wang, C., et al. (1998). Cardiac graft intercellular adhesion molecule-1 (ICAM-1) and interleukin-1 expression mediate primary isograft failure and induction of ICAM-1 in organs remote from the site of transplantation. Circ. Res. 82, 762-772.
- Xu, H., et al. (1994). Leukocytosis and resistance to septic shock in intercellular adhesion molecule 1-deficient mice. J. Exp. Med. 180, 95-109.
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