Advanced
Part of Thermo Fisher Scientific
Thermo Scientific - Pierce Protein Research Products  

Document Center

  Certificate of Analysis
  Product Instructions
  MSDS
  Print This Page
  Request A Catalog

User Profile

Email Address:
Password:
 
  I Forgot My Password
  Register
  Why Register?

Shopping Cart

  Ordering Information
Shipping & Handling
Security Info
Returns Info
  Current Order
Cart is Empty
Quick Order



Email Sign-up Email Sign-up

Subscribe to our RSS feed!  RSS



 


YOU ARE HERE:  Browse Product Catalog > Western Blotting, ELISA and Cell Imaging > Nucleic Acid Labeling and Detection > North2South Chemiluminescent Detection Kits  


North2South Chemiluminescent Detection of Nucleic Acids 

High sensitivity Southern blotting without radioactivity.

North2South Kit for Southern blotting and DNA fingerprinting.
Detection of biotinylated DNA on a Southern blot using the North2South System and a cooled CCD imaging system. A Southern blot of biotinylated DNA samples from a DNA fingerprinting kit was detected using the North2South System and imaged for one minute using a cooled CCD camera system.

The North2South Hybridization and Detection Kit combines a novel enhanced luminol substrate for horseradish peroxidase (HRP) with optimized pre-made hybridization and blocking buffers to ensure consistent results and high sensitivity. Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe. After a brief membrane blocking step, the biotin label is detected with streptavidin- HRP and a 5-minute incubation with the chemiluminescent substrate. Results are easily recorded by brief exposure to X-ray film (1-15 minutes) or CCD camera.


Features

  • Sensitivity equal to or greater than 32P
  • Much faster than DIG detection systems
  • Amenable to nearly any DNA or RNA hybridization method
  • Optimized hybridization and blocking buffers and protocol steps yield consistent results from experiment to experiment
  • High level of light output and consistent low background allows for very short and multiple exposure times using cooled CCD camera systems or film
  • Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user
  • Specific plaques can be picked out of areas that are very dense with signal due to extremely low background

Related Resources and Products:

North2South Chemiluminescent Kit FAQ
LightShift Chemiluminescent EMSA Kit

References:

  1. Adilakshmi, T. and Laine, R.O. (2002). J. Biol. Chem. 277(6), 4147-4151.
  2. Bach, S., et al. (2002). Infect. Immun. 70(2), 988-992.
  3. Borst, E-M., et al. (1999). J. Virol. 73(10), 8320-8329.
  4. Haertel-Wiesmann, M., et al. (2000). J. Biol. Chem. 275(41), 32046-32051.

Ordering Information
Certificate of AnalysisCertificate of Analysis   Product InstructionsProduct Instructions   MSDSMSDS
Buy Product # Description Pkg. Size Files Price
Add 17097 North2South Chemiluminescent Hybridization and Detection Kit
Sufficient reagents for 1,000 cm2 membranes.
Kit contains:
Hybridization Buffer 125 ml
2X Hybridization Stringency Wash Buffer 375 ml
Blocking Buffer 500 ml
4X Wash Buffer 500 ml
Substrate Equilibration Buffer 500 ml
Stabilized Streptavidin-HRP Conjugate 1.5 ml
Luminol/Enhancer Solution   80 ml
Stable Peroxide Solution   80 ml
Kit Product Instructions MSDS for product # 17097 North2South Chemiluminescent Hybridization and Detection Kit $402.00
Add 17175 North2South Complete Biotin Random Prime Labeling and Detection Kit
Kit contains:
Product #17097 - North2South Chemiluminescent Nucleic Acid Hybridization and Detection Kit.
This kit contains substrate, buffers and stabilized Streptavidin-Horseradish Peroxidase sufficient for 1,000 cm2 of membrane.

Product #17075 North2South Biotin Random Prime Kit.
This kit contains reagents sufficient for 10 biotinylation reactions.

Kit Product Instructions $515.00
Add 17295 North2South Chemiluminescent Substrate for HRP
Sufficient substrate for 1,000 cm2 of membrane.
Kit contains:
Chemiluminescent Substrate:
North2South Luminol/Enhancer Solution  50 ml

North2South Stable Peroxide Solution

50 ml
Kit Product Instructions MSDS for product # 17295 North2South Chemiluminescent Substrate for HRP $275.00
Add 37549 North2South Hybridization Buffer 125 ml MSDS for product # 37549 North2South Hybridization Buffer $111.00
Add 37555 North2South Hybridization Stringency Wash Buffer (2X) 375 ml MSDS for product # 37555 North2South Hybridization Stringency Wash Buffer (2X) $100.00

Learn more about other Thermo Scientific Life Science Research Products
Biopolymers Cell Culture High Content Screening  
Nucleic Acid Technologies PCR Reagents & Plastics RNAi Technologies  European Sales

 © 2010 Thermo Fisher Scientific Inc.

PO Box 117, Rockford, IL 61105 USA
1-800-874-3723  or  815-968-0747

Trademarks Privacy Statement