An extra-mild tissue cell lysis solution designed for total protein extraction from tissue samples.

Thermo Scientific T-PER Tissue Protein Extraction Reagent was developed for mild extraction of total protein from tissue samples. This tissue cell lysis reagent utilizes a proprietary detergent in 25mM bicine, 150mM sodium chloride (pH 7.6) to maximize the efficiency of protein solubilization from mammalian tissue samples by homogenization. The simple composition is compatible with additives such as protease inhibitors, salts, reducing agents, chelating agents, providing versatility for many different sample types and lysis applications. Cell lysates prepared with T-PER Reagent are directly compatible with reporter assays (e.g., luciferase, beta-galactosidase, chloramphenicol acetyl transferase), protein kinase assays (e.g., PKA, PKC, tyrosine kinase), immunoassays (e.g., Western blots, ELISAs, RIAs) and/or protein purification procedures.

Highlights:

• Simple procedure – just homogenize tissue sample in 1:20 (w/v) of tissue to T-PER Reagent, then centrifuge to pellet cell or tissue debris
• Mild and convenient – detergent is dialyzable for quick and easy removal; can be used with additional components (e.g., protease inhibitors, salts, reducing agents, chelating agents, etc.)
• Flexible – lysate may be used for reporter assays, protein kinase assays, immunoassays and/or protein purifications
• Compatible – the lysate is compatible with standard protein assays such as Thermo Scientific Pierce Coomassie Plus (Bradford) Protein Assay

Product Details:

Biological tissue is a series of interconnected cells that perform a similar function within an orgnanism. To further analyze the function of the tissue and each cell within, it is necessary to lyse the cells and study the individual proteins. This can be difficult as tissue is quite dense and especially with interconnections between cells. Methods of tissue and cellular disruption require the use of physical means such as mortar and pestle, shearing, sonication and processors. Tissue can also be manipulated by temperature or freeze/thawing which causes the cell adhesion properties to weaken and allow for cell lysis into buffer. Physical disruption of tissue at cold temperatures (0 to 4°C) helps to slow down protein degradation and keep some protein interactions intact. Regardless of the method chosen for cellular disruption, each method can be easily facilitated using the T-PER Tissue Protein Extraction Reagent.

References:

1. Aldred, M.A., et al. (2003). Cancer Res. 63, 2864-2871.
2. Chakravarti, S. et al. (2003). Invest. Ophth. & Vis. Sci., 44(6), 2422-2432.
3. Jepsen, K.H., et al. (2002). J. Biol. Chem. 277, 35532-35540.
4. Lukashevich, I.S., et al. (2003). J. Virol. 77(3), 1727-1737.
5. Paweletz, C., et al. (2001). Oncogene 20, 1981 - 1989.
6. Runkuan, Y., et al. (2002). Am J Physiol Gastrointest Liver Physiol 283, G212–G221
7. Runkuan, Y., et al. (2002). Amer. J. Pathol. 161, 1475-1484.
8. Sheng, J.G., et al. (2002). J. Neurosci. 22(22), 9794-9799.
9. Smallwood, A, et al. (2003). Biol. of Reprod. 69, 286–293.
10. Yan, W. et al. (2003). PNAS 100(12), 7189-7194.
11. Zen, Y. et al. (2002). American Journal of Pathology 161(4), 1475 - 1484,

Related Products:
Mammalian Protein Extraction Reagent
Nuclear and Cytoplasmic Extraction Reagents
Organelle Enrichment Kits
Eukaryotic Membrane Protein Extraction Kit
Mitochondria Isolation Kit for Cultured Cells
Halt Protease Inhibitor Cocktail

Related Links:
Pierce Cell Lysis Handbook
Remove detergent from protein samples
Cell Lysis Technical Information