An efficient, ready-to-use mammalian cell lysis solution that eliminates scraping, freeze-thaw and sonication methods.

When conducting protein research studies, protein extraction is usually the first key step in the analysis procedure. To extract protein from cultured cells it is necessary to perform cell lysis, thus opening the cell and releasing the proteins of interest. Several methods are commonly used to physically lyse cells, including mechanical disruption, liquid homogenization, sonication, freeze/thaw cycles and manual grinding.

Mammalian cells are some of the simplest cell types to lyse and to accomplish successful cell lysis, Thermo Scientific offers a detergent based protein extraction buffer that leaves protein in its native conformation. The M-PER Mammalian Protein Extraction Reagent uses a non-denaturing detergent to quickly extract total cellular protein in only 5 minutes. Lysis with M-PER Reagent is so efficient that adherent cells do not need to be scraped from the culture dish, enabling easy lysis, collection and analysis of proteins from cells grown in 24-well and 96-well plates.

M-PER prepares a total cell lysate (works with adherent and suspension cells) that is compatible with many downstream assays including immunoassays, enzyme assays and a variety of common reporter assays. The detergent is so mild that each cell lysate is immediately compatible with Coomassie (Bradford) and BCA Protein Assays for protein quantitation without dilution or protein precipitation. Furthermore, significantly more protein can be obtained with this method compared with freeze/thaw and sonication. Choose the complete cell lysis reagent with a detergent formulation that dissolves cell membranes at low concentrations, does not denature protein and is compatible with downstream assays.

Figure 2. M-PER Reagent compatibility with reporter assays in transiently transfected mammalian cells. Mammalian FM2 cells were transiently transfected with a reporter construct containing the luciferase gene. The transfected cells were lysed with either M-PER Reagent or Brand P lysis buffer and subjected to luciferase assay. For b-galactosidase and CAT assays, MDA-MB-231 cells were contransfected with reporter constructs expressing  b-galactosidase and CAT, respectively. The transfected cells were lysed with M-PER Reagent or the freeze/thaw method, and the lysates were assayed for  b-galactosidase and CAT activity.

• Lyse adherent cells directly in plate or after scraping and washing in suspension
• Mild detergent lysis, yielding extracts that are immediately compatible with Coomassie (Bradford) and BCA Protein Assays or SDS-PAGE
• Extracts soluble proteins in nondenatured state, enabling direct use in immunoprecipitation and other affinity purification procedures
• Amine-free and fully dialyzable formulation ensures compatibility with subsequent assay systems
• Maintain luciferase, b -galactosidase, CAT and other reporter gene activities as well or better than competitor products and freeze/thaw methods

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Ordering Information	Certificate of Analysis   Product Instructions   MSDS
Buy Product # Description Pkg. Size Files Price Add 78501 M-PER Mammalian Protein Extraction Reagent 250 ml $234.00 Add 78503 M-PER Mammalian Protein Extraction Reagent 25 ml $59.00 Add 78505 M-PER Mammalian Protein Extraction Reagent 1000 ml $634.00