A simple apparatus for simultaneously dialyzing 12 small-volume samples using flat sheets of dialysis membrane.

The Thermo Scientific Pierce Microdialyzer System 100 is an apparatus that offers a quick, simple method for small-volume sample desalting or buffer exchange.

The Microdialyzer System consists of an acrylic device designed to hold a sheet of pre-framed dialysis membrane (sold separately) so that as many as 12 samples (each 20 to 100µL) can be dialyzed against a constant flow of buffer. The unit is equipped with a lid to prevent evaporation of the sample, and a unique inlet/outlet system for easy buffer exchange. The design minimizes protein loss and provides excellent sample recovery. A continual flow of buffer can be used to maintain a very high concentration gradient and achieve very rapid, uniform and complete dialysis.

After clamping one of several available Thermo Scientific Pre-Framed Dialysis Membranes into the manifold and then filling the buffer chamber, samples are simply pipetted into the wells for the procedure. When dialysis is complete, the samples are easily removed by pipetting. Pre-Framed Dialysis Membranes are available with several molecular-weight cutoff (MWCO) specifications, enabling the dialysis conditions to be customized for very specific experimental needs.

Highlights: Validated for use with compatible pre-framed dialysis sheets, available in five molecular-weight cutoff designations: 1K, 3K, 8K, 20K and 50K Integrated lid eliminates the possibility of evaporation during dialysis Dialysate chamber includes a magnetic stir bar to increase dialysis efficiency Reusable and replaceable valves and fittings enable adaptation to a variety of uses One-way valve system provides efficient regulation of buffer exchange Design allows access to dialysate chamber and channels air bubbles to a vent valve Applications: Purifying membrane proteins Removing protein modification reagents and crosslinkers Removing oligosaccharides from protein solutions Dialyzing prior to electrophoresis Exchanging, collecting, and/or analyzing buffer during dialysis

Procedure summary for the Microdialyzer System 100.