D-Salt Polyacrylamide Desalting Columns are pre- packed with porous polyacrylamide beads. The D-Salt Polyacrylamide gel is not subject to enzymatic degradation and will not serve as a nutrient for microbial growth. The gel is very hydrophilic, so there should be little interaction between the gel and sample molecules. Contamination with low molecular weight sugars (which may occur with crosslinked dextran) is not a concern when using D-Salt Polyacrylamide gel. Oxidizing agents can be removed without destroying the support. This gel is susceptible to hydrolysis of amide groups under extreme pH conditions, so an operating pH of 2-10 is recommended at room temperature.

The D-Salt Polyacrylamide gel can also be autoclaved at pH 5.5-6.5 for 30 minutes at 120°C.D-Salt Polyacrylamide 1,800 Desalting Columns have a wet bead diameter of 45-90 µm. These columns are ideal for separating lower molecular weight compounds with a fractionation range of 100-1,800. D-Salt Polyacrylamide 6,000 gel is available in either plastic or glass columns with exclusion limits of 6,000 molecular weight. Both types of columns display excellent chromatographic properties.

Gravity-flow Gel Filtration involves the chromatographic separation of molecules of different dimensions based on their relative abilities to penetrate into a suitable stationary phase. A chromatographic matrix, usually consisting of very small, uncharged porous particles in an aqueous solution, is packed into a column and then used for the separation. Different levels of separation can be achieved based on the pore size of the medium packed into the column. The medium can be chosen to totally exclude proteins or large molecules, while still including small solutes. Large molecules are excluded from the internal pores of the gel and emerge first from the column. The smaller molecules are able to penetrate the pores, then progress through the column at a slower rate. These smaller molecules are subsequently flushed through the column with additional buffer volume.

Desalting and buffer exchange are two of the most widely used applications of gel filtration chromatography.

Highlights:

• Polyacrylamide gel is stable in water, salt solutions, organic solvents and alkaline or acidic conditions.
• Excellent flow properties
• Heat-stable

Applications:

• Removing salts from protein solutions
• Removing phenol from nucleic acid preparations
• Separating excess crosslinker from conjugate preparations
• Removing excess derivatizing agents from modified proteins
• Removing unreacted dye from fluorescent antibodies
• Removing free radiolabel from labeled proteins
• Exchanging one buffer for another

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