D-Salt Excellulose Desalting Columns are pre-packed with small, porous cellulose beads. These columns have a wet bead diameter of 40-100 µm and an exclusion limit of 5,000 molecular weight. Excellulose gel is stable in water, salt solutions, organic solvents and alkaline or acidic conditions. It also demonstrates excellent mechanical stability and offers excellent sample recoveries as a result of the slower flow rate. 

Gravity-flow Gel Filtration involves the chromatographic separation of molecules of different dimensions based on their relative abilities to penetrate into a suitable stationary

phase. A chromatographic matrix, usually consisting of very small, uncharged porous particles in an aqueous solution, is packed into a column and then used for the separation. Different levels of separation can be achieved based on the pore size of the medium packed into the column. The medium can be chosen to totally exclude proteins or large molecules, while still including small solutes. Large molecules are excluded from the internal pores of the gel and emerge first from the column. The smaller molecules are able to penetrate the pores, then progress through the column at a slower rate. These smaller molecules are subsequently flushed through the column with additional buffer volume.

Desalting and buffer exchange are two of the most widely used applications of gel filtration chromatography.

Highlights:

• Excellulose gel is stable in water, salt solutions, organic solvents and alkaline or acidic conditions.
• Excellent mechanical stability
• Excellent sample recoveries

Applications:

• Removing salts from protein solutions
• Removing phenol from nucleic acid preparations
• Separating excess crosslinker from conjugate preparations
• Removing excess derivatizing agents from modified proteins
• Removing unreacted dye from fluorescent antibodies
• Removing free radiolabel from labeled proteins
• Exchanging one buffer for another

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