Highlights:

• Specific stain for use with SDS-PAGE to detect abundant phosphoprotein components of the sample
• Easy-to-follow protocol yields results in three hours
• Phosphoproteins stain in-gel as green to-green-blue bands
• Detects the phosphoproteins phosvitin and β-casein in the 40 ng-80 ng/ band and 80 ng-160 ng/band range, respectively
• Kit includes one positive control protein (phosvitin) and one negative control protein (soybean trypsin inhibitor)
• Specific stain for use with SDS-PAGE to detect phosphoprotein (phosphoserine and phosphothreonine residues) components of the sample
• Phosphoprotein-stained gels can be stained with GelCode™ Blue Stain Reagent (Product # 24590, 24592) for total protein profiling

    GelCode Phosphoprotein Stain                    GelCode Blue Stain

Figure 1. GelCode Phosphoprotein Stain specifically detects phosphorylated proteins.
An identical selection of proteins was run on two gels and stained with either GelCode
Phosphoprotein Stain or GelCode Blue Stain for total protein detection. A: soybean trypsin
inhibitor (negative control), B: bovine serum albumin, C: phosvitin, D: histone III-S, E: ovalbumin,
F: β-casein and G: prestained protein molecular weight markers.

Applications:

• Use to evaluate the progress of a phosphoprotein purification
• Excellent potential for use in dephosphorylation studies

Figure 2. GelCode Phosphoprotein Stain staining protocol.

Figure 3. GelCode Phosphoprotein Stain destaining protocol.

References:

1. Simpson, R.J. (2003). Proteins and Proteomics. A Laboratory Manual. Cold Spring Harbor Laboratory Press. pp. 201-202.