Detect His-tagged proteins directly in gel.

You may never need to perform a costly and time consuming western blotting step again to verify 6xHis tagged expressed protein. GelCode 6xHis Protein Tag Staining Kit…another Pierce first!
Unique features offer exceptional benefits to life scientists expressing fusion protein with the popular 6xHis tag!

Figures 1 and 2. Detection of 6xHis-tagged proteins in E. coli lysates. Lysates of three E. coli preparations expressing different histidine-tagged proteins and one control E. coli lysate. A. Gel stained with GelCode 6xHis Protein Tag Staining Kit. B. Same gel stain with GelCode Blue Stain Reagent.

Highlights:

• Fluorescent detection is designed to be specific for histidine-tagged proteins
• Detects directly on the gel – eliminates the need for membrane transfer and Western blot detection
• 2-3x faster than western blotting. Get results sooner and save hours of valuable research time
• All reagents are ready-to-use. No reagents to prepare or mix or dilute prior to use.
• Only 2 reagents required. Guarantees a simple to perform, mistake-free detection protocol
• Fluorescent detection designed to be specific for 6xHis tagged proteins only
• Protein can be transferred after staining allowing Western-based detection, if necessary
• Stain compatible with the use of Pierce GelCode Blue Stain Reagent. Stain for 6xHis tagged protein specifically and follow with a general Coomassie stain, such as GelCode Blue Stain Reagent for total protein profile determination.
• Detects down to 5.7 picomoles histidine-tagged protein with CCD camera and 57 picomoles using a transilluminator
• Compatible with GelCode Blue Stain Reagent – stain for histidinetagged protein specifically and follow with GelCode Blue Stain Reagent for a total protein profile determination