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 E-Zinc Reversible Stain Kit 
A reversible, rapid zinc stain that has excellent sensitivity equal to that of silver stain.

When you need results quickly, E-Zinc Stain can be used to stain a gel in just 15 minutes. The sensitivity is similar to silver staining – but without the tedious staining process. Best of all, the E-Zinc Stain doesn’t require the proteins to be fixed in the gel, so the proteins are not altered. That means you can stain a gel before transferring for Western blot or analyzing by mass spectrometry.

GelCode E-Zinc Reversible Stain
Figure 1. E-Zinc Reversible Stain.
Gels viewed and photographed with white light against blue background.

Highlights:

Sensitive – see as little as 0.25 ng of protein
• Fast – results in 15 minutes
• All components are ready to use
• No fixing of gel required
• Develops opaque white background while protein bands remain clear

Useful staining strategy for:

  • Protein recovery for antibody generation or immunological detection
  • Protein/peptide recovery from gel for sequencing purposes
  • Protein digest sequencing by mass spectrometry
  • Biological enzyme activity assays
  • Western blots (pre- or post-transfer)
  • Quick purity checks before proceeding with purification or transfer for Western blotting

Reversibility of stain allows:

  • Alternative staining of same gel
  • Protein elution or transfer after gel staining and destaining

GelCode E-Zinc staining protocol
Figure 2. E-Zinc staining protocol.

GelCode E-Zinc destaining protocol
Figure 3. E-Zinc destaining protocol.

References:

  1. Amano, S., et al. (2000). J. Biol. Chem. 275, 22728-22735.
  2. Steiglitz, B.M., et al. (2002). J. Biol. Chem. 277, 49820-49830.


Ordering Information
Bulk quantities: InquireBulk quantities: Inquire    Certificate of AnalysisCertificate of Analysis   Product InstructionsProduct Instructions   MSDSMSDS
Product # Description Pkg. Size Files Price
24582 E-Zinc Reversible Stain Kit
Sufficient for staining up to 20 SDS-PAGE 8 cm x 10 cm mini gels.
Kit contains:
E-Zinc Stain
500 ml
E-Zinc Developer500 ml
E-Zinc Eraser500 ml
Kit Product Instructions MSDS for product # 24582 E-Zinc Reversible Stain Kit $108.00


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