Color Silver Stained SDS-PAGE gel

Highlights:

• Designed for one- and two-dimensional PAGE gel staining
• Total time, after fixing, varies from 11-90 minutes (depending on gel thickness)
• Sensitivity as low as 0.1 ng protein/mm2 in the gel
• Detects proteins that do not bind silver as yellow spots
• Proteins stain in five basic colors: black, blue, brown, red and yellow
• Four simple staining steps
• No stop baths or reversers needed
• Stains proteins, DNA, lipids and polysaccharides

Color aids in protein mapping by:

• Distinguishing overlapping spots
• Identifying post-translationally modified proteins
• Tracking proteins in biological fluids
• Monitoring the alteration of proteins in disease states
• Monitoring the subcellular fractions of cells

Color Silver Stain protocol.
Basic protocol, after gel fixing step, based on 0.75 mm gel.

References:

1. Sammons, D.W., et al. (1981). Electrophoresis 2, 135-141.
2. Ochs. D.C., et al. (1981). Electrophoresis 2, 304-307.
3. Dunbar, B.D., et al. (1983). Electrophoresis 4, 258-259.
4. Schoenle, E.J., et al. (1984). J. Biol. Chem. 259, 12112-12116.
5. Sammons, D.W., et al. (1984). Two-Dimensional Gel Electrophoresis-Proteins. Eds.
   Celis, J.E. and Bravo, R.. Academic Press. pp. 111-126.
6. Slisz, M.L. and Van Frank, R.M. (1985). Electrophoresis 6, 405-408.
7. Dunbar, B.S., et. al. (1990). Methods Enzymol. 182, 441-459.
8. Sammons, D.W., et. al. (1981). Electrophoresis 2, 141-147.
9. Stoppler, H., et. al. (1997). J. Biol. Chem. 272, 13332-13337.