Advanced
Part of Thermo Fisher Scientific
Thermo Scientific - Pierce Protein Research Products  

Document Center

  Certificate of Analysis
  Product Instructions
  MSDS
  Print This Page
  Request A Catalog

User Profile

Email Address:
Password:
 
  I Forgot My Password
  Register
  Why Register?

Shopping Cart

  Ordering Information
Shipping & Handling
Security Info
Returns Info
  Current Order
Cart is Empty
Quick Order



Email Sign-up Email Sign-up

Subscribe to our RSS feed!  RSS



 


YOU ARE HERE:  Browse Product Catalog > Protein Electrophoresis and Staining > Staining Kits and Reagents > Metal Based Stains > Color Silver Stain Kit  


Color Silver Stain Kit 
The only silver staining system offering quantitative, reproducible color staining.

GelCode Color Silver Stained SDS-PAGE gel
Color Silver Stained SDS-PAGE gel

Highlights:

  • Designed for one- and two-dimensional PAGE gel staining
  • Total time, after fixing, varies from 11-90 minutes (depending on gel thickness)
  • Sensitivity as low as 0.1 ng protein/mm2 in the gel
  • Detects proteins that do not bind silver as yellow spots
  • Proteins stain in five basic colors: black, blue, brown, red and yellow
  • Four simple staining steps
  • No stop baths or reversers needed
  • Stains proteins, DNA, lipids and polysaccharides

Color aids in protein mapping by:

  • Distinguishing overlapping spots
  • Identifying post-translationally modified proteins
  • Tracking proteins in biological fluids
  • Monitoring the alteration of proteins in disease states
  • Monitoring the subcellular fractions of cells

Figure 1. GelCode Color Silver Stain protocol. Basic protocol, after gel fixing step, based on 0.75 mm gel
Color Silver Stain protocol.
Basic protocol, after gel fixing step, based on 0.75 mm gel.

References:

  1. Sammons, D.W., et al. (1981). Electrophoresis 2, 135-141.
  2. Ochs. D.C., et al. (1981). Electrophoresis 2, 304-307.
  3. Dunbar, B.D., et al. (1983). Electrophoresis 4, 258-259.
  4. Schoenle, E.J., et al. (1984). J. Biol. Chem. 259, 12112-12116.
  5. Sammons, D.W., et al. (1984). Two-Dimensional Gel Electrophoresis-Proteins. Eds.
    Celis, J.E. and Bravo, R.. Academic Press. pp. 111-126.
  6. Slisz, M.L. and Van Frank, R.M. (1985). Electrophoresis 6, 405-408.
  7. Dunbar, B.S., et. al. (1990). Methods Enzymol. 182, 441-459.
  8. Sammons, D.W., et. al. (1981). Electrophoresis 2, 141-147.
  9. Stoppler, H., et. al. (1997). J. Biol. Chem. 272, 13332-13337.


Ordering Information
Certificate of AnalysisCertificate of Analysis   Product InstructionsProduct Instructions   MSDSMSDS
Buy Product # Description Pkg. Size Files Price
Add 24597 Color Silver Stain Kit

Sufficient reagent to stain up to 25 (18 x 18 cm)
2-D gels or 40 (10 x 13 cm)2-D gels.
Kit contains:
Silver Reagent500 ml
Reducer Base 500 ml
Reducer Aldehyde500 ml
Stabilizer Base 500 ml
Kit Product Instructions MSDS for product # 24597 Color Silver Stain Kit $201.00


Related Items 
Downloadable Files
GelCode Color Silver Stain Kit FAQ
Learn more about other Thermo Scientific Life Science Research Products
Biopolymers Cell Culture High Content Screening  
Nucleic Acid Technologies PCR Reagents & Plastics RNAi Technologies  European Sales

 © 2010 Thermo Fisher Scientific Inc.

PO Box 117, Rockford, IL 61105 USA
1-800-874-3723  or  815-968-0747

Trademarks Privacy Statement