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 SDS-Out SDS Precipitation Reagent 

The solution to small-sample SDS removal for downstream analysis.

Thermo Scientific SDS-Out SDS Precipitation Reagent provides convenient removal of SDS from microliter-volume samples. The simple reagent and kit provide for effective removal of excess SDS from small protein samples. Sodium dodecyl sulfate (SDS) is a detergent typically used in cell lysis reagents and other protein solubilization buffers. Traditional methods for SDS removal include prolonged dialysis, anion exchange chromatography, affinity columns or and acetone precipitation. By comparison, the SDS-Out Precipitation Reagent is relatively easy to use and provides excellent protein recovery.

Highlights of SDS-Out SDS Precipitation Reagent:

  • Convenient removal of SDS from microliter-volume samples
  • Use for samples containing 0.1 to 1% SDS
  • Minimal dilution of small-volume samples
  • Protein recovery is 90 to 100% (based on tests with BSA, cytochrome C, soybean trypsin inhibitor, ovalbumin, ribonuclease A, myoglobin and human IgG)

 

Protein recovery after removal of SDS from protein solution with increasing SDS concentrations using Thermo Scientific SDS-Out SDS Precipitation Reagent.
  Protein*  Protein Recovery
Bovine Serum Albumin 97.5% 98.0% 98.4% 100%
Cytochrome C 100% 100% 100% 100%
Soybean Trypsin Inhibitor 99.0% 98.5% 98.8% 100%
Ovalbumin 99.8% 99.6% 98.1% 93.8%
Ribonuclease 100% 100% 100% 100%
Myoglobin 100% 100% 100% 97.9%
Human IgG 100% 92.7% 88.7% 76.2%
Initial Concentration of SDS 0.125% 0.25% 0.5% 1.0%
* 0.5mL samples of protein solution each at a concentration of 1mg/mL were used to develop this table.

 

Related Products and Resources:

Pierce Detergent Removal Spin Columns
Browse Thermo Scientific Pierce Detergents
Protein Methods Library: Detergents for Cell Lysis



Ordering Information
Bulk quantities: InquireBulk quantities: Inquire    Certificate of AnalysisCertificate of Analysis   Product InstructionsProduct Instructions   MSDSMSDS
Product # Description Pkg. Size Files Price
20308 SDS-Out SDS Precipitation Kit
Sufficient For: Precipitation of SDS from 200mL of protein solution
Kit Contents:
SDS Precipitation Reagent, 10mL
Spin Cup Columns, 12 columns
Collection Tubes, 12 tubes
10mL kit Product Instructions MSDS for product # 20308 SDS-Out SDS Precipitation Kit $143.00

20310 SDS-Out SDS Precipitation Reagent
Formulation: Proprietary
Sufficient For: Precipitation of SDS from 500mL of protein solution
25mL Product Instructions MSDS for product # 20310 SDS-Out SDS Precipitation Reagent $142.00


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