The solution to small-sample SDS removal for downstream analysis.
Thermo Scientific SDS-Out SDS Precipitation Reagent provides convenient removal of SDS from microliter-volume samples. The simple reagent and kit provide for effective removal of excess SDS from small protein samples. Sodium dodecyl sulfate (SDS) is a detergent typically used in cell lysis reagents and other protein solubilization buffers. Traditional methods for SDS removal include prolonged dialysis, anion exchange chromatography, affinity columns or and acetone precipitation. By comparison, the SDS-Out Precipitation Reagent is relatively easy to use and provides excellent protein recovery.
Highlights of SDS-Out SDS Precipitation Reagent:
- Convenient removal of SDS from microliter-volume samples
- Use for samples containing 0.1 to 1% SDS
- Minimal dilution of small-volume samples
- Protein recovery is 90 to 100% (based on tests with BSA, cytochrome C, soybean trypsin inhibitor, ovalbumin, ribonuclease A, myoglobin and human IgG)
| Protein recovery after removal of SDS from protein solution with increasing SDS concentrations using Thermo Scientific SDS-Out SDS Precipitation Reagent.
|
| Protein* |
Protein
Recovery |
| Bovine Serum Albumin |
97.5% |
98.0% |
98.4% |
100% |
| Cytochrome C |
100% |
100% |
100% |
100% |
| Soybean Trypsin Inhibitor |
99.0% |
98.5% |
98.8% |
100% |
| Ovalbumin |
99.8% |
99.6% |
98.1% |
93.8% |
| Ribonuclease |
100% |
100% |
100% |
100% |
| Myoglobin |
100% |
100% |
100% |
97.9% |
| Human IgG |
100% |
92.7% |
88.7% |
76.2% |
| Initial Concentration of SDS |
0.125% |
0.25% |
0.5% |
1.0% |
| * 0.5mL samples of protein solution each at a concentration of 1mg/mL were used to develop this table. |
Related Products and Resources:
Pierce Detergent Removal Spin Columns
Browse Thermo Scientific Pierce Detergents
Protein Methods Library: Detergents for Cell Lysis
|
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