Electrophoresis and lysis buffer grade SDS formulation.

Thermo Scientific Sodium Dodecyl Sulfate (Lauryl) is standard-grade SDS detergent for use in protein polyacrylamide gel electrophoresis (PAGE). This lauryl-grade sodium dodecyl sulfate (SDS) is a popular anionic detergent for routine protein electrophoresis and cell lysis methods. The formulation is a mixture of several different alkyl sulfate chain lengths (C10 to C18).

Highlights of Sodium Dodecyl Sulfate (SDS):

• Popular anionic detergent for a variety of protein methods
• Especially useful for denaturing polyacrylamide gel electrophoresis (SDS-PAGE)
• Common component of cell lysis buffers

Properties of SDS (values for pure C₁₂):

• Molecular Weight: 288.5g
• Detergent Class: Ionic (anionic)
• Aggregation Number: 62
• Micelle Molecular Weight: 18,000g
• Critical Micelle Concentration (CMC): 6 to 8mM (0.1728 to 0.2304%, w/v)
• Cloud Point: >100°C
• Dialyzable: No

Specifications for Sodium Dodecyl Sulfate (Part No. 28364, 28365):

• Visual: White powder, free of foreign material.
• Solubility: 10% (aq, w/v) solution must be clear, colorless to slightly yellow.
• Chain length: C12 >60%; C14 = 20 to 35%; C16 <10%; C10 and C18 <1% each

Specifications for SDS, 20% Solution (Part No. 62202):

• Visual: Clear, colorless liquid, free of foreign material.
• pH: 5 to 8
• DNase, RNase and Protease: None

Related Products and Resources:

Detergent Sampler Pack
Browse all Thermo Scientific Pierce Detergents
Protein Methods Library: Detergents for Cell Lysis