Advanced
Part of Thermo Fisher Scientific
Thermo Scientific - Pierce Protein Research Products  
Brij-35 Detergent Solutions
Brij-58 Detergent Solution
CHAPS & CHAPSO
n-Dodecyl-beta-D-Maltoside
NP-40 Detergent Solution
Octyl-beta-Glucoside
Octyl-beta-Thioglucopyranoside
SDS (Sodium Dodecyl Sulfate-C12)
SDS (Sodium Dodecyl Sulfate-Lauryl)
Sodium Cholate
Sodium Deoxycholate
Surfact-Amps Detergent Sampler
Triton X-100 Detergent Solution
Triton X-114 Detergent Solution
Tween-20 Detergent Solution
Tween-80 Detergent Solution

Document Center

  Certificate of Analysis
  Product Instructions
  MSDS
  Request a Catalog

User Profile

Email Address:
Password:
 
  Forgot Password
  Register
  Why Register?

Shopping Cart

 Cart is Empty
 Quick Order
  Ordering Information
Shipping & Handling
Returns Info
Security Info
Terms & Conditions



Email Sign-up  Email Sign-up

Subscribe to our RSS feed!  RSS

Follow us on Facebook  Follow Us


 





Detergents for Cell Lysis 

High-purity, low-peroxide, surfactant solutions and detergent solids for use in cell lysis reagent formulation, protein solubilization procedures, wash buffers for ELISA, and other protein research methods.


Brij-35 is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or a surfactant in various HPLC applications. 
 

Brij-58 is a nonionic polyoxyethylene surfactant detergent supplied in an easy-to-use 10% (w/v) purified solution for use in routine and high-demand protein molecular biology techniques and HPLC applications. 
 

A non-denaturing detergent able to disrupt nonspecific protein interactions and has less protein aggregation than non-ionic detergents. It is electrically neutral and easily removed by dialysis.  
 

n-Dodecyl-ß-D-Maltoside is especially useful for solubilizing membrane proteins to preserve their activity. The water soluble nonionic detergent is most often used for the isolation of hydrophobic membrane proteins. 
 

Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized and supplied as a 10% solution in 10mL glass ampules. 
 

Octyl beta-Glucoside is a non-ionic detergent that has been widely used for membrane protein solubilization. This detergent is dialyzable but, unlike OTG, is susceptible to beta-Glucosidase enzymatic activity. 
 

Octylthioglucoside (OTG) is a low-MW nonionic detergent that is effective for cell lysis and nondenaturing protein solubilization. It is resistant to beta-D-glucoside glucohydrolase degradation, unlike Octyl-beta-glucoside. 
 

Sodium Dodecyl Sulfate (C12) is a highly purified form of SDS that is especially suited for protein experiments requiring tightly controlled solubility parameters. Sodium dodecyl sulfate (SDS) is an alkyl sulfate, anionic detergent used frequently in prot 
 

Unique distribution of carbon chain lengths in sequanal grade SDS (lauryl) is advantageous in resolving viral proteins during gel electrophoresis.  
 

Sodium cholate is a water-soluble, ionic detergent used for cell lysis, liposome preparation, isolation of membrane proteins and lipids. Used for preventing nonspecific binding in affinity chromatography and as a cell culture media supplement. 
 

Sodium Deoxycholate is an water soluble, ionic detergent used for disrupting protein interactions, as a component of cell lysis buffers (e.g., RIPA buffer), liposome preparation, isolating membrane proteins and a cell culture media supplement. 
 

Surfact-Amps Detergent Sampler is a convenient collection of 7 detergent solutions and 3 detergent solids useful in protein research methods. An economical way to test detergent effectiveness for a particular protein storage or experimental system. 
 

Surfact-Amps X-100 Detergent Solution is highly-purified Triton X-100 detergent stabilized and supplied as a 10% solution in 10mL glass ampules. 
 

Surfact-Amps X-114 Detergent Solution is highly-purified Triton X-114 detergent stabilized and supplied as a 10% solution in 10mL glass ampules. 
 

Surfact-Amps 20 Detergent Solution is highly-purified Tween-20 detergent stabilized and supplied as a 10% solution in 10mL glass ampules. 
 

Surfact-Amps 80 Detergent Solution is highly-purified Tween-80 detergent stabilized and supplied as a 10% solution in 10mL glass ampules. 
 

Learn more about other Thermo Scientific Life Science Research Products
Biopolymers Cell Culture High Content Screening  
Nucleic Acid Technologies PCR Reagents & Plastics RNAi Technologies  European Sales

 © 2010 Thermo Fisher Scientific Inc.

PO Box 117, Rockford, IL 61105 USA
1-800-874-3723  or  815-968-0747

Trademarks Privacy Statement