Advanced
Part of Thermo Fisher Scientific
Thermo Scientific - Pierce Protein Research Products  

Document Center

  Certificate of Analysis
  Product Instructions
  MSDS
  Print This Page
  Request A Catalog

User Profile

Email Address:
Password:
 
  I Forgot My Password
  Register
  Why Register?

Shopping Cart

  Ordering Information
Shipping & Handling
Security Info
Returns Info
  Current Order
Cart is Empty
Quick Order



Email Sign-up Email Sign-up

Subscribe to our RSS feed!  RSS

 

YOU ARE HERE:  Browse Product Catalog > Crosslinking and Protein Modification > Reducing Agents > Reduce-Imm Reducing Columns  


Reduce-Imm Reducing Columns 
Solid phase system for reducing peptides and proteins.



 Reduce-Imm Immobilized Reductant Pre-packed Column

Both the mercaptan and the DTT methods of reduction use soluble reducing agents. This requires that the reducing reagents be removed from the sample before further procedures can be performed. Measurements of free sulfhydryl content cannot be made in the presence of these agents, and they must be removed prior to crosslinking. Removal of a soluble reductant can be difficult when a peptide is involved, and extensive desalting may result in protein loss or extreme dilution when small quantities of protein are reduced. Reduce-Imm Reducing Columns were developed to eliminate these difficulties, and are highly effective for reducing disulfide bonds. Immobilized TCEP Disulfide Reducing Gel is also available for reduction of peptides and proteins without contaminating your sample with reducing agent.

Highlights:

  • Eliminates soluble components which interfere with the assay of free thiol groups
  • No need to remove reducing agent after the reaction
  • Reduced protein, free of contaminating thiols, can be easily assayed using the BCA method
  • Solid phase; protocols for both peptides and proteins
  • Excellent protein recovery
  • Regenerable matrix useable up to 4 times

Related Products
Immobilized TCEP Disulfide Reducing Gel
No-Weigh DTT
 

Ordering Information
 Certificate of AnalysisCertificate of Analysis   Product InstructionsProduct Instructions   MSDSMSDS
Buy Product # Description Pkg. Size Files Price
Add 77701 Reduce-Imm Immobilized Reductant Column 1 x 2 ml Product Instructions MSDS for product # 77701 Reduce-Imm Immobilized Reductant Column $184.00

Learn more about other Thermo Scientific Life Science Research Products
Biopolymers Cell Culture High Content Screening  
Nucleic Acid Technologies PCR Reagents & Plastics RNAi Technologies  European Sales

 © 2010 Thermo Fisher Scientific Inc.

PO Box 117, Rockford, IL 61105 USA
1-800-874-3723  or  815-968-0747

 Trademarks Privacy Statement